Re: A question about KO# Biology - 生物学
M*e
1 楼
windysea, it seems that you have to do KO job as well. good:).
typical design for the targeting construct will aim at the
first several exons. in my case, since i only got the genomic
fragment in the middle, and several years ago mouse genome
sequence was not published yet, i had to choose a functional
domain that is important to the gene, in my case, a protein
kinase. when i got the knockouts, the phenotype was pretty
interesting so that i could get some publications; however,
i still could not
typical design for the targeting construct will aim at the
first several exons. in my case, since i only got the genomic
fragment in the middle, and several years ago mouse genome
sequence was not published yet, i had to choose a functional
domain that is important to the gene, in my case, a protein
kinase. when i got the knockouts, the phenotype was pretty
interesting so that i could get some publications; however,
i still could not