Re: Questions about JC-1 staining# Biology - 生物学
J*n
1 楼
The emmission wavelength of JC-1 is 590, it is orange (for most people).
For ideal flow cytometry, you have to change your filter system to get
optimal result. For confocal microscopy, try to put in approiate filter
(usually it's hard), or you might need to do some real good compensation
(sometime
difficult, too). So, the third choise for confocal will be monitoring only one
channel (decrease of orange or increase of green)
If the nuclear is stained, that means your JC-1 concentration in the cel
For ideal flow cytometry, you have to change your filter system to get
optimal result. For confocal microscopy, try to put in approiate filter
(usually it's hard), or you might need to do some real good compensation
(sometime
difficult, too). So, the third choise for confocal will be monitoring only one
channel (decrease of orange or increase of green)
If the nuclear is stained, that means your JC-1 concentration in the cel