Re: some silly questions about microarray# Biology - 生物学
h*b
1 楼
There are primarily 2 types of microarray, comercial Affymetrix chip and
spotted array(cDNA/pcr), I guess u mean spotted array.
two major differences between the 2 types:
1. spotted material: affy use de novo synthesized oligo, 20mer or 25 mer,
called a probe, each gene is represented by 11? or 16 probes. the whole thing
called a probeset, so a probeset correspond to a gene. spotted array is
relative flexible, can print on the chip cDNA(comercially synthesized) or pcr
product, or even promoter s
spotted array(cDNA/pcr), I guess u mean spotted array.
two major differences between the 2 types:
1. spotted material: affy use de novo synthesized oligo, 20mer or 25 mer,
called a probe, each gene is represented by 11? or 16 probes. the whole thing
called a probeset, so a probeset correspond to a gene. spotted array is
relative flexible, can print on the chip cDNA(comercially synthesized) or pcr
product, or even promoter s