y*2
2 楼
一句话说就是死水里起了波澜,然而已经沸腾的岩浆终究没有引起火山爆发的故事。
人物感情刻画非常之细腻,唯一感觉遗憾的就是念白有些那个时代的烙印,略感苍
白。
也有人将玉纹的命运比喻为中国在百废待兴时期要做的艰难的选择,颇有见地。
btw,1950年前的电影各位有那些推荐? 中外都可以。
人物感情刻画非常之细腻,唯一感觉遗憾的就是念白有些那个时代的烙印,略感苍
白。
也有人将玉纹的命运比喻为中国在百废待兴时期要做的艰难的选择,颇有见地。
btw,1950年前的电影各位有那些推荐? 中外都可以。
k*i
3 楼
先谢谢各位看官!
俺想知道有没有谁做IHC的时候没有perfusion,直接处理的fresh tissue?
谢谢啦
俺想知道有没有谁做IHC的时候没有perfusion,直接处理的fresh tissue?
谢谢啦
c*7
4 楼
fleamarket, someone can deposit RMB in chinese bank account within a day.
s*e
5 楼
all depends on how big is your tissue
I rarely perfuse embryonic tissue
I rarely perfuse embryonic tissue
w*4
6 楼
我在美国的账户是chase的,可以直接用chase转账吗?
h*o
7 楼
I was under the impression that perfusion is mainly to get rid of the blood
or more specifically IgGs out of the tissue so there will be much less
background in IHC.
Correct me if I am wrong
For embryos, endogenous IgGs are relatively low compared to adult tissues.
Maybe that's why it works well without perfusion.
【在 s******e 的大作中提到】
: all depends on how big is your tissue
: I rarely perfuse embryonic tissue
or more specifically IgGs out of the tissue so there will be much less
background in IHC.
Correct me if I am wrong
For embryos, endogenous IgGs are relatively low compared to adult tissues.
Maybe that's why it works well without perfusion.
【在 s******e 的大作中提到】
: all depends on how big is your tissue
: I rarely perfuse embryonic tissue
k*a
8 楼
west union
w*4
10 楼
都听说west union比较贵
k*i
13 楼
我是觉得如果直接frozen tissue,剩下的tissue还可以做别的protein assay。要是
perfusion的话,就不能了。
perfusion的话,就不能了。
w*4
14 楼
是啊,还是去chase问问,谢谢
w*s
15 楼
perfusion with saline or PFA?
saline is to remove the blood from the tissue; and PFA is to fix the large
tissue that takes a long time to be fixed by immersion.
correct me if i'm wrong.
saline is to remove the blood from the tissue; and PFA is to fix the large
tissue that takes a long time to be fixed by immersion.
correct me if i'm wrong.
n*w
16 楼
先saline把血洗了,然后perfuse with pfa。你会很明显地观察到一些特征表明各处基
本都fix了。。。这时候可以停了,取器官。。。
本都fix了。。。这时候可以停了,取器官。。。
D*a
17 楼
小鼠大脑切片,反正我们做的明显perfusion的比直接扔pfa的好看。
相关阅读
如何使用addgene购买质粒求一篇文献,谢谢求文章:Fueling the flame:bioenergy couples metabolism and inflammation改名字上法庭要注意什么?李援朝的千人计划太恶心,让米国千老无出头之日zt申请NIH GRANT 对TITLE有什么要求吗听说NIH现在是正式员工工资减10%,上班时间也减少10%求教Lentivirus shRNA knock-down实验求篇全文,万分感谢!有没有蛋白本身是高度ubiquitinated的?paper help, Many thankspaper help!Varshavsky 为啥没拿到炸药生物土博紧急求助(转载)什么杨的paper会被retracted呀?一般生物PI会提前多久通知博后走人? (转载)miRNA qPCR 求推荐便宜可靠试剂盒。菜鸟好奇请问NIH funding 问题 (转载)一“神”人University of Florida农学系的offer怎么还没下来啊!