g*d
2 楼
娱乐圈早已不像之前那样对于明星的恋爱太计较,很多明星都公布了自己的恋情
。甚至直接生娃,生完娃继续回归娱乐圈,还是继续火。
但是,这种一般都是成名之后才开始恋爱的。像杨紫这样作为成人的明星,才开
始火就公布恋情,对于自身其实没有多少好处。本来杨紫在娱乐圈的地位根本没有奠定
,现在说她是几线都太早,她还需要历练和增加人气。
这种人气让她能够达到一种地位,不要像何洁那样,曾经很火,现在结婚复出后
基本成了跑龙套。另外,明星太年轻谈恋爱很不现实,毕竟处于成长期,以后说不定千
差万别,差距太大又怎能在一起。邓紫棋和林宥嘉就是他们以后的结局。
。甚至直接生娃,生完娃继续回归娱乐圈,还是继续火。
但是,这种一般都是成名之后才开始恋爱的。像杨紫这样作为成人的明星,才开
始火就公布恋情,对于自身其实没有多少好处。本来杨紫在娱乐圈的地位根本没有奠定
,现在说她是几线都太早,她还需要历练和增加人气。
这种人气让她能够达到一种地位,不要像何洁那样,曾经很火,现在结婚复出后
基本成了跑龙套。另外,明星太年轻谈恋爱很不现实,毕竟处于成长期,以后说不定千
差万别,差距太大又怎能在一起。邓紫棋和林宥嘉就是他们以后的结局。
w*1
3 楼
Wed, 18 Jul 2012 11:49:44 PDT
Following last week's launch of a hack that allowed users to obtain In App
Purchase content free of charge by routing their purchase requests through a
server run by a Russian hacker, Apple began taking steps to thwart the
method. The hacker has, however, continued to develop his method to skirt
around Apple's roadblocks.
One of the suggestions for a method by which Apple could improve the
security of In App Purchasing was to include a unique identifier in
validation receipts, and we've received word that developers are now seeing
something along those lines coming from receipts issued by Apple since late
yesterday. The receipts carry a new field called "unique_identifer" that
appears to include the Unique Device Identifier (UDID) for the device making
the In App Purchase.
As one developer noted to us, apps are no longer supposed to be collecting
the UDID and thus it is unclear whether Apple's use of the identifier for
this purpose is simply a first step toward a broader implementation of
unique receipt identifiers for increased security or if Apple is attempting
to identify those users and devices who are sharing their receipts with the
Russian hacker to allow the method to function.
Following last week's launch of a hack that allowed users to obtain In App
Purchase content free of charge by routing their purchase requests through a
server run by a Russian hacker, Apple began taking steps to thwart the
method. The hacker has, however, continued to develop his method to skirt
around Apple's roadblocks.
One of the suggestions for a method by which Apple could improve the
security of In App Purchasing was to include a unique identifier in
validation receipts, and we've received word that developers are now seeing
something along those lines coming from receipts issued by Apple since late
yesterday. The receipts carry a new field called "unique_identifer" that
appears to include the Unique Device Identifier (UDID) for the device making
the In App Purchase.
As one developer noted to us, apps are no longer supposed to be collecting
the UDID and thus it is unclear whether Apple's use of the identifier for
this purpose is simply a first step toward a broader implementation of
unique receipt identifiers for increased security or if Apple is attempting
to identify those users and devices who are sharing their receipts with the
Russian hacker to allow the method to function.
g*i
4 楼
上一台还是大一时候买的t60,后来坏掉了换了mac,操控方面感觉mbp的多点触摸比小
红点好用太多了,
现在想再买台thinkpad搞仿真使,现在它上边的触摸板支持多点触摸了么?能自定义三
指或四指的各种指令么?
红点好用太多了,
现在想再买台thinkpad搞仿真使,现在它上边的触摸板支持多点触摸了么?能自定义三
指或四指的各种指令么?
V*b
5 楼
我现在发现一个蛋白(Nrf1)的mRNA水平在我的癌细胞里是正常细胞的10倍。我该如何
去寻找哪个或者哪些转录因子负责Nrf1的转录调控呢?有几个方案,大家看看行不行:
1. 做Nrf1的promoter序列分析,看看有哪些转录因子可以和这个promoter序列结合。
2. 筛选法:把可能性比较大的转录因子一个个knockdown,然后看哪个负责Nrf1的转录
调控。
3. 钓鱼法:做shRNA screen -- transcript chip
我做这个比较外行,想听听大家的意见。
去寻找哪个或者哪些转录因子负责Nrf1的转录调控呢?有几个方案,大家看看行不行:
1. 做Nrf1的promoter序列分析,看看有哪些转录因子可以和这个promoter序列结合。
2. 筛选法:把可能性比较大的转录因子一个个knockdown,然后看哪个负责Nrf1的转录
调控。
3. 钓鱼法:做shRNA screen -- transcript chip
我做这个比较外行,想听听大家的意见。
s*x
6 楼
Nothing you can do... This is quite common.
n*y
7 楼
建议继续用mac
d*7
10 楼
找gene expression array data,看那些TF的expression和你这个有correlation,找
出一些可能性大的,然后用你所说的第2种方法试试
有钱的话直接siRNA screen
出一些可能性大的,然后用你所说的第2种方法试试
有钱的话直接siRNA screen
b*8
12 楼
我觉得这个有两种思路,
1)构建Nrf1基因的启动子序列截断性报告基因质粒,垂钓出参与调控Nrf1转录的关键
顺式元件,再进一步结合EMSA/ChIP等找出转录因子。这个应该是比较常规的做法,很
多paper尤其是JBC上面的很多paper都用这个套路。
2)做promoter magnetic precipitation (PMP) coupled with mass spectrometry.具
体方法请参考这篇paper,http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19015635
祝好运。
1)构建Nrf1基因的启动子序列截断性报告基因质粒,垂钓出参与调控Nrf1转录的关键
顺式元件,再进一步结合EMSA/ChIP等找出转录因子。这个应该是比较常规的做法,很
多paper尤其是JBC上面的很多paper都用这个套路。
2)做promoter magnetic precipitation (PMP) coupled with mass spectrometry.具
体方法请参考这篇paper,http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19015635
祝好运。
V*b
14 楼
多谢。第一个方法有文献参考吗?
【在 b**********8 的大作中提到】
: 我觉得这个有两种思路,
: 1)构建Nrf1基因的启动子序列截断性报告基因质粒,垂钓出参与调控Nrf1转录的关键
: 顺式元件,再进一步结合EMSA/ChIP等找出转录因子。这个应该是比较常规的做法,很
: 多paper尤其是JBC上面的很多paper都用这个套路。
: 2)做promoter magnetic precipitation (PMP) coupled with mass spectrometry.具
: 体方法请参考这篇paper,http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19015635
: 祝好运。
【在 b**********8 的大作中提到】
: 我觉得这个有两种思路,
: 1)构建Nrf1基因的启动子序列截断性报告基因质粒,垂钓出参与调控Nrf1转录的关键
: 顺式元件,再进一步结合EMSA/ChIP等找出转录因子。这个应该是比较常规的做法,很
: 多paper尤其是JBC上面的很多paper都用这个套路。
: 2)做promoter magnetic precipitation (PMP) coupled with mass spectrometry.具
: 体方法请参考这篇paper,http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&dopt=Citation&list_uids=19015635
: 祝好运。
b*8
16 楼
You may refer to this study
http://www.ncbi.nlm.nih.gov/entrez/query.fcgicmd=Retrieve&db=Pu
http://www.jbc.org/content/284/51/35725.full.pdf
【在 V***b 的大作中提到】
: 多谢。第一个方法有文献参考吗?
X*n
17 楼
Yeast One Hybrid?
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