j*8
2 楼
如持 AP&EAD Combo Card入境, 是否意为着使用了EAD?
g*g
3 楼
辛晓琪原唱
俩俩相忘是93版《倚天屠龙记》的片尾曲之一
能在2/10 或是2/12 最好。
每个人5个包子(俺现在木有, 到时候会搞到)。
也提前祝大家金蛇狂歌!!!
拈朵微笑的花
想一番人世变换
到头来输赢又何妨
日与月互消长
富与贵难久长
今早的容颜老于昨晚
眉间放一字宽
看一段人世风光
谁不是把悲喜在尝
海连天走不完
恩怨难计算
昨日非今日该忘
浪滔滔人渺渺
青春鸟飞去了
纵然是千古风流浪里摇
风潇潇人渺渺
快意刀山中草
爱恨的百般滋味随风飘
眉间放一字宽
看一段人世风光
谁不是把悲喜在尝
海连天走不完
恩怨难计算
昨日非今日该忘
浪滔滔人渺渺
青春鸟飞去了
纵然是千古风流浪里摇
风潇潇人渺渺
快意刀山中草
爱恨的百般滋味随风飘
眉间放一字宽
看一段人世风光
谁不是把悲喜在尝
海连天走不完
恩怨难计算
昨日非今日该忘
俩俩相忘是93版《倚天屠龙记》的片尾曲之一
能在2/10 或是2/12 最好。
每个人5个包子(俺现在木有, 到时候会搞到)。
也提前祝大家金蛇狂歌!!!
拈朵微笑的花
想一番人世变换
到头来输赢又何妨
日与月互消长
富与贵难久长
今早的容颜老于昨晚
眉间放一字宽
看一段人世风光
谁不是把悲喜在尝
海连天走不完
恩怨难计算
昨日非今日该忘
浪滔滔人渺渺
青春鸟飞去了
纵然是千古风流浪里摇
风潇潇人渺渺
快意刀山中草
爱恨的百般滋味随风飘
眉间放一字宽
看一段人世风光
谁不是把悲喜在尝
海连天走不完
恩怨难计算
昨日非今日该忘
浪滔滔人渺渺
青春鸟飞去了
纵然是千古风流浪里摇
风潇潇人渺渺
快意刀山中草
爱恨的百般滋味随风飘
眉间放一字宽
看一段人世风光
谁不是把悲喜在尝
海连天走不完
恩怨难计算
昨日非今日该忘
s*j
4 楼
年轻的mm啊,太幼稚
不幼稚点的啊,又嫌大了
不幼稚点的啊,又嫌大了
s*s
5 楼
以前记得是找个transposon里面的RE site, 再找一个frequent cutter,
切下来然后circlization,PCR测序。这个方法效率如何?有没有更加方
便的方法?怎么想这个circlization不怎么靠谱
切下来然后circlization,PCR测序。这个方法效率如何?有没有更加方
便的方法?怎么想这个circlization不怎么靠谱
E*1
6 楼
你俩名字相同性别相同大概就可以。
d*u
7 楼
no
w*w
8 楼
嗯。好听的歌~~
期待~~
期待~~
z*x
11 楼
EAD does not serve as an reentry visa.
l*e
12 楼
贴个老师的:
i*n
13 楼
这说明lz你跟我一样,还是太较真了
其实有句话说的话,关了灯都一样。。。
其实有句话说的话,关了灯都一样。。。
x*e
14 楼
tail-pcr, inverse-pcr would be more efficient.
y*r
15 楼
no!
Baozi
Baozi
f*a
18 楼
要破产咯。。
l*1
23 楼
linker-based PCR
plus
iMapper soft:
//www.ncbi.nlm.nih.gov/pubmed/18974167
//geocachingsoftware.com/imapper.html
more details
please to to E-Book
Chapter 2:
its link:
FTP://ftp.sanger.ac.uk/pub4/theses/kong/chapter2.pdf
citation:
>Based on these expectations, a web-based server called iMapper (Insertional
Mutagenesis Mapping and Analysis Tool) was developed for the efficient
analysis of insertion site sequence reads against vertebrate and
invertebrate Ensembl genomes. Taking linker-based PCR sequence reads as
input, iMapper first scans the sequence to identify a tag sequence (TS)
derived from the end of the insertional mutagen using a local sequence
alignment (LSA) algorithm. iMapper then scans the downstream sequence for
user defined contaminating sequences, then processes the sequences to
identify the restriction site sequence used for
linker ligation during the insertion site PCR, clips out the genomic
sequence between the tag sequence and first restriction enzyme cutting site
and presents this sequence to a rapid mapping algorithm called SSAHA (102).
Insertion sites can then be navigated in Ensembl in the context of other
genomic features such as gene structures. iMapper also generates FASTA and
GFF files of the clipped sequence reads and provides a graphical overview of
the mapped insertion sites against a karyotype. iMapper is designed for
high-throughput applications and can efficiently process tens of thousands
of DNA sequence reads in a short time.
and
its References
No. 102:
//ukpmc.ac.uk/articles/PMC311141/
that Transposons E- Book
full text
FTP://ftp.sanger.ac.uk/pub4/theses/kong/abstract.pdf
FTP site //ftp.sanger.ac.uk/pub4/theses/kong/chapter1.pdf
FTP site //ftp.sanger.ac.uk/pub4/theses/kong/chapter3.pdf
FTP site //ftp.sanger.ac.uk/pub4/theses/kong/chapter4.pdf
FTP site //ftp.sanger.ac.uk/pub4/theses/kong/chapter5.pdf
FTP site //ftp.sanger.ac.uk/pub4/theses/kong/chapter6.pdf
FTP://ftp.sanger.ac.uk/pub4/theses/kong/references.pdf
FTP://ftp.sanger.ac.uk/pub4/theses/kong/appendix.pdf
original posting:
http://www.mitbbs.com/article_t/Biology/31647911.html
18th
【在 s******s 的大作中提到】
: 以前记得是找个transposon里面的RE site, 再找一个frequent cutter,
: 切下来然后circlization,PCR测序。这个方法效率如何?有没有更加方
: 便的方法?怎么想这个circlization不怎么靠谱
plus
iMapper soft:
//www.ncbi.nlm.nih.gov/pubmed/18974167
//geocachingsoftware.com/imapper.html
more details
please to to E-Book
Chapter 2:
its link:
FTP://ftp.sanger.ac.uk/pub4/theses/kong/chapter2.pdf
citation:
>Based on these expectations, a web-based server called iMapper (Insertional
Mutagenesis Mapping and Analysis Tool) was developed for the efficient
analysis of insertion site sequence reads against vertebrate and
invertebrate Ensembl genomes. Taking linker-based PCR sequence reads as
input, iMapper first scans the sequence to identify a tag sequence (TS)
derived from the end of the insertional mutagen using a local sequence
alignment (LSA) algorithm. iMapper then scans the downstream sequence for
user defined contaminating sequences, then processes the sequences to
identify the restriction site sequence used for
linker ligation during the insertion site PCR, clips out the genomic
sequence between the tag sequence and first restriction enzyme cutting site
and presents this sequence to a rapid mapping algorithm called SSAHA (102).
Insertion sites can then be navigated in Ensembl in the context of other
genomic features such as gene structures. iMapper also generates FASTA and
GFF files of the clipped sequence reads and provides a graphical overview of
the mapped insertion sites against a karyotype. iMapper is designed for
high-throughput applications and can efficiently process tens of thousands
of DNA sequence reads in a short time.
and
its References
No. 102:
//ukpmc.ac.uk/articles/PMC311141/
that Transposons E- Book
full text
FTP://ftp.sanger.ac.uk/pub4/theses/kong/abstract.pdf
FTP site //ftp.sanger.ac.uk/pub4/theses/kong/chapter1.pdf
FTP site //ftp.sanger.ac.uk/pub4/theses/kong/chapter3.pdf
FTP site //ftp.sanger.ac.uk/pub4/theses/kong/chapter4.pdf
FTP site //ftp.sanger.ac.uk/pub4/theses/kong/chapter5.pdf
FTP site //ftp.sanger.ac.uk/pub4/theses/kong/chapter6.pdf
FTP://ftp.sanger.ac.uk/pub4/theses/kong/references.pdf
FTP://ftp.sanger.ac.uk/pub4/theses/kong/appendix.pdf
original posting:
http://www.mitbbs.com/article_t/Biology/31647911.html
18th
【在 s******s 的大作中提到】
: 以前记得是找个transposon里面的RE site, 再找一个frequent cutter,
: 切下来然后circlization,PCR测序。这个方法效率如何?有没有更加方
: 便的方法?怎么想这个circlization不怎么靠谱
f*a
26 楼
现在到处都是点歌的包子,hoho, 先吃大的。
E*t
30 楼
袜子断我财路
w*w
36 楼
哎~~小波咋不都贴出来,懒人一个~~
http://www.mitbbs.com/article/Music/31589987_3.html
【在 z******g 的大作中提到】
: 袜子当时挖坟的时候一定是漏掉了,
: 话说就是光明弟先我一步唱的,
: 我的伴奏还是光明弟给的呢,在此谢过!
http://www.mitbbs.com/article/Music/31589987_3.html
【在 z******g 的大作中提到】
: 袜子当时挖坟的时候一定是漏掉了,
: 话说就是光明弟先我一步唱的,
: 我的伴奏还是光明弟给的呢,在此谢过!
E*t
37 楼
囧rz
w*w
38 楼
嗯,需要激励一下子~~
s*e
42 楼
小声问下,包子啥时发?
g*g
45 楼
谁能告诉我, 该给谁发包子。
w*w
46 楼
帮顶~~
快来领包子
快来领包子
w*w
50 楼
呵呵,恭喜了~~
g*g
51 楼
看一段人世风光
谁不是把悲喜在尝
富与贵难久长
到头来输赢又何妨
恩怨难计算
昨日非今日该忘
爱恨的百般滋味随风飘
Well Said, Well Said.
谁不是把悲喜在尝
富与贵难久长
到头来输赢又何妨
恩怨难计算
昨日非今日该忘
爱恨的百般滋味随风飘
Well Said, Well Said.
g*g
54 楼
纪念那逝去的一切。
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