[合集] 请问saponin透化细胞做免疫染色有什么好处？# Biology - 生物学
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bewithu (x-fire in the sea) 于 (Sun Dec 31 10:38:41 2006) 提到:
For regular immunofluorescence, Trition may be good enough (such as 0.1% in
PBS, 10-15 min, RT). Saponin, which is generally purified from plants, is a
much weaker detergent. It is believed that the permeabilzation effect given
by saponin is reversible, thus you need to use saponin-containing solutions
for all steps during IF (Ab incubation and washing).
The advantage is that saponin will gi
bewithu (x-fire in the sea) 于 (Sun Dec 31 10:38:41 2006) 提到:
For regular immunofluorescence, Trition may be good enough (such as 0.1% in
PBS, 10-15 min, RT). Saponin, which is generally purified from plants, is a
much weaker detergent. It is believed that the permeabilzation effect given
by saponin is reversible, thus you need to use saponin-containing solutions
for all steps during IF (Ab incubation and washing).
The advantage is that saponin will gi