Re: questions about DNA and hybridization,thanks a lot~ - 未名空间MITBBS历史存档

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Re: questions about DNA and hybridization,thanks a lot~

Re: questions about DNA and hybridization,thanks a lot~# Biology - 生物学

M*e2002-04-23 07:04

1 楼

For such microarray analysis, typically the mRNA is reverse-
transcribed into complementary DNA (cDNA) and labeled with
fluorescence dye or radioisotope. and the single-stranded
cDNA is hybridized to the complementary strand that is already
spread on the matrix with known position. i have only used
microarray from Clontech. theoretically, the RT enzyme with
gene-specific primers can reverse-transcribe the mRNA into
same amount of cDNA. otherwise, you cannot make the comparison
quantitatively.
fo

l*k2002-04-23 07:04

2 楼

the central doctrin of genetics dictates
DNA-> RNA -> protein, though RNA -> DNA sometimes.
for the gene on DNA to work, they first has to be transcripted into RNA, then
in most cases, be translated into Protein. Upregulation means more
transcription of the gene into its corresponder mRNA... downregulation means
the opposite. Gene amplification and deletion happens but very rare, and, most
likely can't be detected by the method you are using.
Gene regulation, as you might have seen, can happen