Re: his-tag蛋白提纯中,equlibrium buffer中的NaCl干什么# Biology - 生物学
u*s
1 楼
My answer for your reference.
Proteins and column both have charges.
Proteins can bind to the column by electrostatic interaction (so-called
non-specific binding). Of course this is not what you expect.
Assume Protein 1 is what you want, protein 2 is impurity.
If there is sodium chloride in the solution, sodium or chloride ion can
compete
with proteins to bind to column. So protein 2 will be removed from the column.
Although protein 1 is also decreased, the specific binding between histadine
and
Proteins and column both have charges.
Proteins can bind to the column by electrostatic interaction (so-called
non-specific binding). Of course this is not what you expect.
Assume Protein 1 is what you want, protein 2 is impurity.
If there is sodium chloride in the solution, sodium or chloride ion can
compete
with proteins to bind to column. So protein 2 will be removed from the column.
Although protein 1 is also decreased, the specific binding between histadine
and