Re: 关于Immunohistochemistry的问题请教！# Biology - 生物学
M*e
1 楼
agree. a good primary is essential to get decent IHC or IF.
typically for dilution of commercial product, i will start
from 1:50-1:500. do not try double labeling with another
antibody unless you are certain that the fluorchrome has
very distince exitation spectrum. frozen sections seem to
be used by Santa Crap all the times for IHC, while they would
tell you that it works for paraformaldehyde-fixed sections
if it indeed works. be aware that the purity of the fixative
(e.g., paraformaldehyde) is
typically for dilution of commercial product, i will start
from 1:50-1:500. do not try double labeling with another
antibody unless you are certain that the fluorchrome has
very distince exitation spectrum. frozen sections seem to
be used by Santa Crap all the times for IHC, while they would
tell you that it works for paraformaldehyde-fixed sections
if it indeed works. be aware that the purity of the fixative
(e.g., paraformaldehyde) is