Re: HELP: how to select RNAi target sequ# Biology - 生物学
n*e
1 楼
There are two very important reports in recent Cell discussing how to design
potent siRNA.One is from Zamore lab, the other from Amgen company. Basically,
the rule is to put a weak base pair, for example A-T or even mismatch with
target, at the 5'-end of the antisense strand of siRNA. Such a design
faciliates RISC selecting the antisense strand as guide strand, instead of
sense strand. Most of previous failed siRNA sequences follow the opposite of
rule. Given this discovery, other considerations
potent siRNA.One is from Zamore lab, the other from Amgen company. Basically,
the rule is to put a weak base pair, for example A-T or even mismatch with
target, at the 5'-end of the antisense strand of siRNA. Such a design
faciliates RISC selecting the antisense strand as guide strand, instead of
sense strand. Most of previous failed siRNA sequences follow the opposite of
rule. Given this discovery, other considerations
