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siRNA vs shRNA?

siRNA vs shRNA?# Biology - 生物学

T*J2011-02-02 08:02

1 楼

在daycare，3岁的女孩又感染Coxsackie 病毒了（ Herpangina），又嘴巴疼的不敢吃
东西，还发烧。不是得过一次就有抗体了吗，怎么又感染了？
5个月前被Coxsackie 病毒弄得高烧，嘴里长小水泡，一周才好点。现在又。。。

j*r2011-02-02 08:02

2 楼

Is there any disadvantage of using siRNA transfection for some functional
assay, such as cell viability or cell death? Can anyone recommend a vendor
for
synthesize dsRNA (if the target sequence is already known for efficient
knockdown, based on the use of shRNA)? Many thanks,

P*s2011-02-02 08:02

3 楼

没听说这个得了以后就可以免疫

【在 T*****J 的大作中提到】

: 在daycare，3岁的女孩又感染Coxsackie 病毒了（ Herpangina），又嘴巴疼的不敢吃
: 东西，还发烧。不是得过一次就有抗体了吗，怎么又感染了？
: 5个月前被Coxsackie 病毒弄得高烧，嘴里长小水泡，一周才好点。现在又。。。

V*f2011-02-02 08:02

4 楼

siRNA transfection only gives transient knockdown (3-7 days). If the
phenotype takes longer than 3-7 days to show, then siRNA hardly works.
Also some cell types are not easy to transfect.
Dharmacon and Ambion are good companies for siRNAs.

n*y2011-02-02 08:02

5 楼

是啊，我娃儿前年夏天连着两次。今年5月应该是又来了一次－－－他就是嘴里长疹子了，手脚上都没。
夏天常见病，没办法

j*r2011-02-02 08:02

6 楼

Thanks. So if I get very quick phenotypes in cultured cells using siRNA
transfection, will the data be convincing? Do I still need stable shRNA (
regular of conditionally if it is lethal) to confirm the results?
It seems there are also disadvantages of shRNA, such as in some cases
sequence-specific toxicity (probably due to overloading the RNAi machineary)
. But I am not sure about the down side of using siRNA.