p*n
2 楼
【 以下文字转载自 Apple 讨论区 】
发信人: JiayiWang (noname), 信区: Apple
标 题: OSX Lion一出，PC时代正式结束了
发信站: BBS 未名空间站 (Wed Oct 20 21:59:23 2010, 美东)
以后的Mobile电脑都会是multi-gesture, no CD no hard-drive, instant on。
除了苹果，市面上没有一个公司能做到这样的硬件，也没有一个这样的操作系统做软件。
微软憋了10年做出的操作系统，刚出来几个月就被苹果踢回上世纪90年代了。
发信人: JiayiWang (noname), 信区: Apple
标 题: OSX Lion一出，PC时代正式结束了
发信站: BBS 未名空间站 (Wed Oct 20 21:59:23 2010, 美东)
以后的Mobile电脑都会是multi-gesture, no CD no hard-drive, instant on。
除了苹果，市面上没有一个公司能做到这样的硬件，也没有一个这样的操作系统做软件。
微软憋了10年做出的操作系统，刚出来几个月就被苹果踢回上世纪90年代了。
n*w
3 楼
Hi, all,
I have a simple question but I do not know the solution...
I am trying to figure out how to do multiple sequence alignment using the
online tool from EMBL.
I always get the warning from the 1st step that at least two sequences are
needed...
I do not know how to fix this since I already put two sequences...
Probably I used the wrong format for that.
Thank you all...
I have a simple question but I do not know the solution...
I am trying to figure out how to do multiple sequence alignment using the
online tool from EMBL.
I always get the warning from the 1st step that at least two sequences are
needed...
I do not know how to fix this since I already put two sequences...
Probably I used the wrong format for that.
Thank you all...
l*y
4 楼
没人理我，自己搞明白了
efile是在网上直接填表，真晕啊
【在 l***y 的大作中提到】
: 求助，刚才填写I-539，最后需要申请人签字。那如何efile呢？签了字再扫描？多谢了
: ！
efile是在网上直接填表，真晕啊
【在 l***y 的大作中提到】
: 求助，刚才填写I-539，最后需要申请人签字。那如何efile呢？签了字再扫描？多谢了
: ！
A*0
5 楼
你有没有用对 file's format?
It works well to me.
【在 n***w 的大作中提到】
: Hi, all,
: I have a simple question but I do not know the solution...
: I am trying to figure out how to do multiple sequence alignment using the
: online tool from EMBL.
: I always get the warning from the 1st step that at least two sequences are
: needed...
: I do not know how to fix this since I already put two sequences...
: Probably I used the wrong format for that.
: Thank you all...
It works well to me.
【在 n***w 的大作中提到】
: Hi, all,
: I have a simple question but I do not know the solution...
: I am trying to figure out how to do multiple sequence alignment using the
: online tool from EMBL.
: I always get the warning from the 1st step that at least two sequences are
: needed...
: I do not know how to fix this since I already put two sequences...
: Probably I used the wrong format for that.
: Thank you all...
n*w
6 楼
That's probably the thing...
Would you mind sending me a sample file? Thank you...
【在 A********0 的大作中提到】
: 你有没有用对 file's format?
: It works well to me.
Would you mind sending me a sample file? Thank you...
【在 A********0 的大作中提到】
: 你有没有用对 file's format?
: It works well to me.
n*w
7 楼
That's probably the thing...
Would you mind sending me a sample file? Thank you...
【在 A********0 的大作中提到】
: 你有没有用对 file's format?
: It works well to me.
Would you mind sending me a sample file? Thank you...
【在 A********0 的大作中提到】
: 你有没有用对 file's format?
: It works well to me.
l*a
8 楼
did you put > at the beginning of each sequencing? It should be like this
>name1
sequence1
>name2
sequence2
....
>name1
sequence1
>name2
sequence2
....
A*0
9 楼
correct. or try this.
>A.DE.x.IFA86_Z30637
ATGGAAAACAGATGGCAGGTGATGATTGTGTGGCAGGTAGACAGGATGAG
GATTAGAACATGGAACAGTTTAGTAAAACATCATATGTATGTCTCTAAGA
AAGCTAAGGGTTGGTTTTATAGACATCACTTTGAATGTAGGCATCCAAAA
GCAAGTTCAGAAGTACACATCCCACTAGGGGATGCTAGATTAGTAGTAAG
AACATATTGGGGTCTGCATACAGGAGAAAGAGACTGGCACTTGGGTCAGG
GGGTCTCCATAGAATGGAGGCAGAAAAGATATAGCACACAAGTAGATCCT
GACCTAGCAGACCAACTGATTCACCTGCATTATTTTGACTGTTTTTCAGA
CTCTGCCATAAGGAAAGCCATATTAGGAGAAGTAGTTAGACCTAGGTGTG
AATATCAAGCAGGACATAACAAGGTAGGATCACTGCAATATTTGGCACTG
AAAGCATTAGTAGCACCAAAAAAGGCAAAGCCACCTTTGCCTAGTCTTAA
GAAGTTAACAGAAGATAGATGGAACGAGCCCCAGAAGACCAGGGGCCACA
GAGGGAACCGTTCAATGAATGGACACTAG
>A.DE.x.K15DE_AF200476
ATGGAAAACAGATGGCAGGTGATGATTGTGTGGCAGGTAGACAGGATGAG
GATTAGAACATGGAACAGTTTAGTAAAACATCATATGTATGTCTCTAAGA
AAGCTAAGGGTTGGTTTTATAGACATCACTTTGAATGTAGGCATCCAAAA
GCAAGTTCAGAAGTACACATCCCACTAGGGGATGCTAGATTAGTAGTAAG
AACATATTGGGGTCTGCATACAGGAGAAAGAGACTGGCACTTGGGTCAGG
GGGTCTCCATAGAATGGAGGCAGAAAAGATATAGCACACAAGTAGATCCT
GACCTAGCAGACCAACTGATTCACCTGTATTATTTTGATTGTTTTTCAGA
CTCTGCCATAAGAAAAGCCATATTAGGAGAAATAGTTAGACCTAGGTGTG
AATATCAAGCAGGACATAATAAGGTAGGATCGCTACAATATTTGGCACTG
AAAGCATTAGTAGCACCAAAAAAGACAAAGCCACCTTTGCCTAGTCTTAA
GAAGTTAGCAGAAGATAGATGGAACAAGCCCCAGAAGACCAGGGGCCACA
GAGGGAACCGTTCAATGAATGGACACTAG
>A.DE.x.K19DE_AF200477
ATGGAAAACAGATGGCAGGTGATGATTGTGTGGCAAGTAGACAGGATGAG
GATTAGAACATGGAACAGTTTAGTAAAACATCATATGTATATCTCAAAGA
AAGCAAAAAATTGGTTTTATAGACATCACTTTGAAAGCAGTCATCCAAGA
GTAAGTTCAGAAGTACACATCCCACTAGGGGATGCTAGATTAGTAGTAAG
AACATATTGGGGTCTGCATACAGGAGAAAAAGATTGGCACTTGGGTAATG
GGGTCTCCATAGAATGGAGACTAAGAAGATATAGCACACAAATAGATCCT
GACCTGGCAGACCAACTAATTCATCTGCATTATTTTAATTGTTTTTCAGA
CTCTGCCATAAGGAAAGCCATATTAGGACAAGTAGTTAGACCTAGGTGTG
ACTATCAAGCAGGACATAACAAGGTAGGATCTCTACAATATTTGGCACTG
AAAGCATTAGTAACACCAGTAAGGACAAGGCCACCTTTGCCTAGTGTTAG
GAAATTAGCAGAGGACAGATGGAACAAGCCCCAGAAGACCAGGGGCCCCA
GAGGGAGCCATACAATGAATGGACATTAG
>A.GE.99.99GEMZ011_DQ207944
ATGGAAAACAGATGGCAGGTGATGATTGTGTGGCAAGTAGACAGGATGAG
GATTAGAACATGGAACAGTTTAGTAAAACATCATATGTATGTCTCAAAGA
AAGCTAGAGAGTGGTTTTATAGACATCACTATGAAAGCAGGCATCCAAAA
ATAAGTTCAGAAGTACACATCCCACTAGGGGATGCTAGGCTAGTAGTAAA
AACATATTGGGGTCTGCATGCAGGAGAAAAAGACTGGCACTTGGGTCATG
GGGTCTCCATAGAATGGAGACAGAAAAGGTATAGCACACAAATAGACCCT
GACCTGGCGGACCAACTAATTCATCTGCATTATTTTGACTGTTTTTCAGA
ATCTGCCATAAGGAAAGCCATAGTAGGACACATAGTTAGCCCTAAGTGTA
ACTATCCACCAGGACACAGCAAGGTAGGATCTCTACAATACTTGGCACTG
AAAGCATTAGTAACACCAACAAGAGAAAGGCCACCTTTGCCTAGTGTTAG
AAAGCTAACAGAGGATAGATGGAACAAGCCCCAGAAGACCAGGGGCCGCA
GAGGGAACCACACAATGAATGGATGTTAG
>A.IN.01.1579A_DQ083238
ATGGAAAACAGATGGCAGGTGATGATTGTGTGGCAGGTAGACAGGATGAG
GATTAGAACATGGCACAGTTTAGTAAAACATCATATGTATGTCTCAAAGA
AAGCAAAAAATTGGGTTTATAAACATCACTTTGAAAGCAGGCATCCAAGA
GTAAGTTCAGAAGTACACATCCCACTAGGAGAAGCTAGATTAGTGGTAAG
AACATATTGGGGTCTGCATACAGGAGAAAAAGACTGGCACTTGGGTCATG
GGGTCTCCATAGAATGGAAGCTAAGAGGATATAACACACAAGTAGATCCT
GACCAGGCAGACCAACTAATTCATCTGCATCATTTTGACTGTTTTTCAGA
CTCTGCCATAAGGAAAGCCATATTAAAACAAGTAGTTAGCCCTAAGTGTG
AATATCAAACAGGACATAACAAGGTAGGATCTCTACAATATTTAGCACTA
AAAGCATTAGTAACACCAACAAAGGCAAAGCCACCTTTGCCTAGTGTTAG
GATATTAGCAGAGGATAGATGGAACAAGCCCCAGAAGACCAGGGGCCCCA
GAGAGAGCCATACAATGAATGGATGTTAG
【在 l*****a 的大作中提到】
: did you put > at the beginning of each sequencing? It should be like this
: >name1
: sequence1
: >name2
: sequence2
: ....
>A.DE.x.IFA86_Z30637
ATGGAAAACAGATGGCAGGTGATGATTGTGTGGCAGGTAGACAGGATGAG
GATTAGAACATGGAACAGTTTAGTAAAACATCATATGTATGTCTCTAAGA
AAGCTAAGGGTTGGTTTTATAGACATCACTTTGAATGTAGGCATCCAAAA
GCAAGTTCAGAAGTACACATCCCACTAGGGGATGCTAGATTAGTAGTAAG
AACATATTGGGGTCTGCATACAGGAGAAAGAGACTGGCACTTGGGTCAGG
GGGTCTCCATAGAATGGAGGCAGAAAAGATATAGCACACAAGTAGATCCT
GACCTAGCAGACCAACTGATTCACCTGCATTATTTTGACTGTTTTTCAGA
CTCTGCCATAAGGAAAGCCATATTAGGAGAAGTAGTTAGACCTAGGTGTG
AATATCAAGCAGGACATAACAAGGTAGGATCACTGCAATATTTGGCACTG
AAAGCATTAGTAGCACCAAAAAAGGCAAAGCCACCTTTGCCTAGTCTTAA
GAAGTTAACAGAAGATAGATGGAACGAGCCCCAGAAGACCAGGGGCCACA
GAGGGAACCGTTCAATGAATGGACACTAG
>A.DE.x.K15DE_AF200476
ATGGAAAACAGATGGCAGGTGATGATTGTGTGGCAGGTAGACAGGATGAG
GATTAGAACATGGAACAGTTTAGTAAAACATCATATGTATGTCTCTAAGA
AAGCTAAGGGTTGGTTTTATAGACATCACTTTGAATGTAGGCATCCAAAA
GCAAGTTCAGAAGTACACATCCCACTAGGGGATGCTAGATTAGTAGTAAG
AACATATTGGGGTCTGCATACAGGAGAAAGAGACTGGCACTTGGGTCAGG
GGGTCTCCATAGAATGGAGGCAGAAAAGATATAGCACACAAGTAGATCCT
GACCTAGCAGACCAACTGATTCACCTGTATTATTTTGATTGTTTTTCAGA
CTCTGCCATAAGAAAAGCCATATTAGGAGAAATAGTTAGACCTAGGTGTG
AATATCAAGCAGGACATAATAAGGTAGGATCGCTACAATATTTGGCACTG
AAAGCATTAGTAGCACCAAAAAAGACAAAGCCACCTTTGCCTAGTCTTAA
GAAGTTAGCAGAAGATAGATGGAACAAGCCCCAGAAGACCAGGGGCCACA
GAGGGAACCGTTCAATGAATGGACACTAG
>A.DE.x.K19DE_AF200477
ATGGAAAACAGATGGCAGGTGATGATTGTGTGGCAAGTAGACAGGATGAG
GATTAGAACATGGAACAGTTTAGTAAAACATCATATGTATATCTCAAAGA
AAGCAAAAAATTGGTTTTATAGACATCACTTTGAAAGCAGTCATCCAAGA
GTAAGTTCAGAAGTACACATCCCACTAGGGGATGCTAGATTAGTAGTAAG
AACATATTGGGGTCTGCATACAGGAGAAAAAGATTGGCACTTGGGTAATG
GGGTCTCCATAGAATGGAGACTAAGAAGATATAGCACACAAATAGATCCT
GACCTGGCAGACCAACTAATTCATCTGCATTATTTTAATTGTTTTTCAGA
CTCTGCCATAAGGAAAGCCATATTAGGACAAGTAGTTAGACCTAGGTGTG
ACTATCAAGCAGGACATAACAAGGTAGGATCTCTACAATATTTGGCACTG
AAAGCATTAGTAACACCAGTAAGGACAAGGCCACCTTTGCCTAGTGTTAG
GAAATTAGCAGAGGACAGATGGAACAAGCCCCAGAAGACCAGGGGCCCCA
GAGGGAGCCATACAATGAATGGACATTAG
>A.GE.99.99GEMZ011_DQ207944
ATGGAAAACAGATGGCAGGTGATGATTGTGTGGCAAGTAGACAGGATGAG
GATTAGAACATGGAACAGTTTAGTAAAACATCATATGTATGTCTCAAAGA
AAGCTAGAGAGTGGTTTTATAGACATCACTATGAAAGCAGGCATCCAAAA
ATAAGTTCAGAAGTACACATCCCACTAGGGGATGCTAGGCTAGTAGTAAA
AACATATTGGGGTCTGCATGCAGGAGAAAAAGACTGGCACTTGGGTCATG
GGGTCTCCATAGAATGGAGACAGAAAAGGTATAGCACACAAATAGACCCT
GACCTGGCGGACCAACTAATTCATCTGCATTATTTTGACTGTTTTTCAGA
ATCTGCCATAAGGAAAGCCATAGTAGGACACATAGTTAGCCCTAAGTGTA
ACTATCCACCAGGACACAGCAAGGTAGGATCTCTACAATACTTGGCACTG
AAAGCATTAGTAACACCAACAAGAGAAAGGCCACCTTTGCCTAGTGTTAG
AAAGCTAACAGAGGATAGATGGAACAAGCCCCAGAAGACCAGGGGCCGCA
GAGGGAACCACACAATGAATGGATGTTAG
>A.IN.01.1579A_DQ083238
ATGGAAAACAGATGGCAGGTGATGATTGTGTGGCAGGTAGACAGGATGAG
GATTAGAACATGGCACAGTTTAGTAAAACATCATATGTATGTCTCAAAGA
AAGCAAAAAATTGGGTTTATAAACATCACTTTGAAAGCAGGCATCCAAGA
GTAAGTTCAGAAGTACACATCCCACTAGGAGAAGCTAGATTAGTGGTAAG
AACATATTGGGGTCTGCATACAGGAGAAAAAGACTGGCACTTGGGTCATG
GGGTCTCCATAGAATGGAAGCTAAGAGGATATAACACACAAGTAGATCCT
GACCAGGCAGACCAACTAATTCATCTGCATCATTTTGACTGTTTTTCAGA
CTCTGCCATAAGGAAAGCCATATTAAAACAAGTAGTTAGCCCTAAGTGTG
AATATCAAACAGGACATAACAAGGTAGGATCTCTACAATATTTAGCACTA
AAAGCATTAGTAACACCAACAAAGGCAAAGCCACCTTTGCCTAGTGTTAG
GATATTAGCAGAGGATAGATGGAACAAGCCCCAGAAGACCAGGGGCCCCA
GAGAGAGCCATACAATGAATGGATGTTAG
【在 l*****a 的大作中提到】
: did you put > at the beginning of each sequencing? It should be like this
: >name1
: sequence1
: >name2
: sequence2
: ....
n*w
10 楼
Thank you all!!!
It worked!!!
It worked!!!
a*y
11 楼
fasta format...
T*t
12 楼
其实FAQ里都应该有吧。。。
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