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怎么知道老鼠background的比例
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怎么知道老鼠background的比例# Biology - 生物学
s*e
1
United States: Visa Office Predicts Significant Movement For EB-2 India &
China; But EB-3 India Stuck In Neutral
05 November 2011
Article by Valentine A. Brown
Last week, Charles Oppenheim, the man behind the mysterious Visa Bulletin
curtain, also known as Chief of the Visa Control and Reporting Division of
the Department of State, made some significant predictions regarding
priority date movement in the coming months.
The best news came for Indian and Chinese nationals in the EB-2 category.
For these individuals, Mr. Oppenheim predicted that priority dates would be
advanced "significantly" over the next few visa bulletins. He indicated that
"significantly" could mean a four month jump to March 1, 2008 for the
December bulletin, and that this trend could continue into the January and
February 2012 bulletins. He also predicted that EB-2 for the Rest of the
World (ROW) category will remain current throughout 2012.
The worst news came for Indian nationals in the EB-3 category. Mr. Oppenheim
predicted that the category will remain stagnant throughout the year. This
is due to the per country limitations placed on each visa category, which
for Indians in EB-3 amounts to only 3000 green cards per year. The queue in
this category is estimated at 210,000, thus making a 70 year wait, "
plausible" according to Oppenheim. There is some hope on the horizon for
persons in EB-3 due to pending legislation, which would eliminate the per
country limitation. See my previous blog on this topic for more information.
Although the news is not as bad for Chinese nationals in EB-3,- a steady
advancement of one to three weeks per month for the next several months - it
still means long waits for visa numbers for applicants in this category.
The wait may be shorter for those EB-3 applicants in the ROW category, as
Oppenheim predicts a 3-4 week movement per month for the next several months
. The chart below demonstrates what these advancements might look like over
the next seven months. Please note that this is only a prediction, but may
help applicants prepare their finances, travel plans and documentation for
possible filing of adjustment of status applications.
Dec '11
Jan '12
Feb '12
Mar '12
Apr '12
May '12
June '12
EB-2 India & China
03/01/2008
07/01/2008
11/01/2008
11/01/2008
11/01/2008
11/01/2008
Possible Retrogression
EB-3 China
09/12/2004
10/3/2004
10/24/2004
11/14/2004
12/5/2004
12/26/2004
01/15/2005
EB-3 India
07/22/2002
07/22/2002
08/01/2002
08/01/2002
08/15/2002
08/15/2002
08/22/2002
EB-3 ROW
01/22/2006
02/15/2006
03/15/2006
04/07/2006
04/21/2006
05/15/2006
06/04/2006
Number crunching by the Visa Office is a difficult business. As explained by
Oppenheim, it is a guessing game for several reasons; (1) it is impossible
to identify the exact number of people in the queue because the number of
spouses and minor children who will be counted against the visa numbers are
unknown until they actually apply for a visa number. This has been
especially the case in EB-3, as the long waits have increased, applicants'
families have grown. (2) Visa numbers are not actually requested until the
end of the green card application process, leaving a 6 month lag time
between priority date movement and actual visa allocation by the State
Department. This problem is what often leads to the retrogression in the
priority dates.
Oppenheim stated that retrogression of EB-2 for India and China is a good
possibility in the Summer of 2012, due to the inexact science of visa number
usage estimation. Oppenheim's theory is that he will advance the numbers
significantly in the early part of the Fiscal Year, develop a queue for visa
numbers, hold the dates steady from March 2012-June 2012, to allow USCIS to
catch up with visa allocation then retrogress in June 2012 or later if
necessary. Even though retrogression is unnerving to those affected by it,
it is a better result than having precious visa numbers unused and then lost
forever if they are not allocated before 9/30/2012. Based upon this
timeline, it is possible that there could be additional EB-2 movement in
September 2012 to ensure that all of the numbers are used before the fiscal
year ends on September 30, 2012.
With the possibility of retrogression, it is important to get I-485
applications filed in the first month of visa availability.
Just like Dorothy in the Wizard of Oz, we have been fooled before by the
Visa Office, so there is no use in getting too excited by the good news, as
it means that certain applicants will be able to file their green card
applications earlier than they might have previously thought. But for those
Indian nationals in EB-3 who have been debating whether to do the necessary
to refile in EB-2, this confirms their worst fears, and suggests that all
steps necessary to get in to EB-2 be initiated, the sooner the better.
We are prepared to handle the anticipated significant movement in the EB-2
China and India Categories. Please contact us if we can be of any assistance.
This article is for general information and does not include full legal
analysis of the matters presented. It should not be construed or relied upon
as legal advice or legal opinion on any specific facts or circumstances.
The description of the results of any specific case or transaction contained
herein does not mean or suggest that similar results can or could be
obtained in any other matter. Each legal matter should be considered to be
unique and subject to varying results. The invitation to contact the authors
or attorneys in our firm is not a solicitation to provide professional
services and should not be construed as a statement as to any availability
to perform legal services in any jurisdiction in which such attorney is not
permitted to practice.
Duane Morris LLP, a full-service law firm with more than 700 attorneys in 24
offices in the United States and internationally, offers innovative
solutions to the legal and business challenges presented by today's evolving
global markets. Duane Morris LLP, a full-service law firm with more than
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administrators and senior managers.
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f*g
2
地理盲制作
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h*n
3
经过好几轮杂交, 想看看一窝小老鼠是否出现了genetic background分离. 各位知道
有什么比较简便的方法吗?
还有, 在多轮杂交的时候, 我觉得每次杂交都必须有一方是单纯genetic background,
这样才不会出现genetic background, 各位觉得对不对.
谢谢指教.
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f*r
4
要过08 12月!

be

【在 s*******e 的大作中提到】
: United States: Visa Office Predicts Significant Movement For EB-2 India &
: China; But EB-3 India Stuck In Neutral
: 05 November 2011
: Article by Valentine A. Brown
: Last week, Charles Oppenheim, the man behind the mysterious Visa Bulletin
: curtain, also known as Chief of the Visa Control and Reporting Division of
: the Department of State, made some significant predictions regarding
: priority date movement in the coming months.
: The best news came for Indian and Chinese nationals in the EB-2 category.
: For these individuals, Mr. Oppenheim predicted that priority dates would be

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f*g
5
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i*0
6
要是都从纯系来可能好估计一点,但是我觉得现在很多老鼠,特别是从合作者要来的的,
都早是mix的了.
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s*e
7
Hope so.
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s*a
8


【在 f*****g 的大作中提到】
: 地理盲制作
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D*a
9
有crossing over,从F2开始都是瞎混的。
就算一方是纯系也一样,混的多少和位点没有两只是一样的
没法查,除非全基因组测一遍
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H*i
10
越前进越好,总比浪费绿卡名额好。

EB-2 India & China
Dec '11---03/01/2008
Jan '12---07/01/2008
Feb '12---11/01/2008
Mar '12---11/01/2008
Apr '12---11/01/2008
May '12---11/01/2008
June '12---Possible Retrogression

【在 s*******e 的大作中提到】
: Hope so.
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e*t
11
多谢,热心人呀
当初我看北唐的时候,是多么希望能有张图看看

【在 f*****g 的大作中提到】

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h*n
12
有没有啥SNP array可以查一下?
我现在做FVB and B6, 如果背景不一样可能reviewer有意见.

【在 D*a 的大作中提到】
: 有crossing over,从F2开始都是瞎混的。
: 就算一方是纯系也一样,混的多少和位点没有两只是一样的
: 没法查,除非全基因组测一遍

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D*a
14
没有任何两只老鼠是一样的,你查了也白查。。。

【在 h********n 的大作中提到】
: 有没有啥SNP array可以查一下?
: 我现在做FVB and B6, 如果背景不一样可能reviewer有意见.

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h*n
15
你是说杂交下来一窝老鼠没有任何两只一样吗? 这个我可以理解.
但是, 比如说, FVB X B6, 我们通常说得到的老鼠50%FVB;50%B6, 类似的情况下, 那么
有没有可能通过查很多gene/microsatelite/SNP看看一个大概的情况, 比如FVB为主还
是B6为主. 我的老鼠的phenotype(metastasis)的程度跟background相关的, 所以我很
关心这个.
谢谢.

【在 D*a 的大作中提到】
: 没有任何两只老鼠是一样的,你查了也白查。。。
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D*a
16
那要查很多吧,工作量很大的。好几百个pcr才能看完一个chromatin好像。花钱肯定会
有人做的,就跟那个快速backcross(号称几代之内得到99% background的某种tg老鼠
)一个原理。
你做开头了,每个实验都要做。
那么就算你做了,你要怎么用这个数据?依据这个把不符合结果的老鼠删掉?或者跟
reviewer说我们这个结果是用treatment解释的,这个结果是用background解释的?
我觉得你的phenotype既然是跟background有关,就不如使劲用一种老鼠当爹(或者妈
),等你实验做到差不多考虑发表的程度,你的纯化background也差不多了。大不了重
复几个开头的实验。
否则你拿F2混交F3,然后F4,然后F5.。。。这样时间长了你的background就固定下来
了。万一你恰好丢了影响你phenotype的那段genome,你就哭吧。。。

【在 h********n 的大作中提到】
: 你是说杂交下来一窝老鼠没有任何两只一样吗? 这个我可以理解.
: 但是, 比如说, FVB X B6, 我们通常说得到的老鼠50%FVB;50%B6, 类似的情况下, 那么
: 有没有可能通过查很多gene/microsatelite/SNP看看一个大概的情况, 比如FVB为主还
: 是B6为主. 我的老鼠的phenotype(metastasis)的程度跟background相关的, 所以我很
: 关心这个.
: 谢谢.

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