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how to determine protein concentration that is bound to lipid membrane???
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how to determine protein concentration that is bound to lipid membrane???# Biology - 生物学
c*4
1
Does anyone know how to measure the concentration of protein that is bound
to lipid membrane? I can not measure it by UV/Vis because the phospholipid
greatly interferences the UV/Vis absorbance at very wide range.
I found a paper that used densitometry with a software (ImageQuant) to
determine the membrane bound protein concentration. Does anyone know how
reliable it would be. Many thanks!!!
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l*a
2
You can use Detergent compatible BCA assay from Biorad. Or Ellman's
reagent for thiol containing protein.

bound
phospholipid
how

【在 c*******4 的大作中提到】
: Does anyone know how to measure the concentration of protein that is bound
: to lipid membrane? I can not measure it by UV/Vis because the phospholipid
: greatly interferences the UV/Vis absorbance at very wide range.
: I found a paper that used densitometry with a software (ImageQuant) to
: determine the membrane bound protein concentration. Does anyone know how
: reliable it would be. Many thanks!!!

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