B*s
2 楼
a*s
3 楼
Need to separate some virions with Sucrose ultracentrifugation (PI said we
don't need to do CsCl :( - that would have been easier to search for
literature if so). Anyway, I did some trial runs. My goal is to separate the
samples to several layers (if there is different shape of viral particles).
Then collect the layers and run on western blot to see and compare.
take I : 17300 rpm at 4C, x 90 min --> no separation
take II: 30000 rpm at 4C, x 90 min --> no separation
take III: 30000 rpm at 4C, x 3 h ---> still no separation
the sucrose pads i used are: 20% (upper), 35% (middle); 50% (bottom); let
sit at 4C overnight before loading samples and ran the program.
total volume 2 ml (each layer 0.5 ml -- including sample layer).
don't need to do CsCl :( - that would have been easier to search for
literature if so). Anyway, I did some trial runs. My goal is to separate the
samples to several layers (if there is different shape of viral particles).
Then collect the layers and run on western blot to see and compare.
take I : 17300 rpm at 4C, x 90 min --> no separation
take II: 30000 rpm at 4C, x 90 min --> no separation
take III: 30000 rpm at 4C, x 3 h ---> still no separation
the sucrose pads i used are: 20% (upper), 35% (middle); 50% (bottom); let
sit at 4C overnight before loading samples and ran the program.
total volume 2 ml (each layer 0.5 ml -- including sample layer).
E*h
4 楼
中国公民乘坐加航航班经加国到达美国或者离开美国,只要有有效中国护照,有效美国
签证,确认的机票,不需要加国签证。
签证,确认的机票,不需要加国签证。
l*h
5 楼
If you calculate the centrifugal force (in g) and know the sedimentation
rate of your virion, you can have an educated guess about the conditions you
need.
the
).
【在 a*****s 的大作中提到】![](/moin_static193/solenoid/img/up.png)
: Need to separate some virions with Sucrose ultracentrifugation (PI said we
: don't need to do CsCl :( - that would have been easier to search for
: literature if so). Anyway, I did some trial runs. My goal is to separate the
: samples to several layers (if there is different shape of viral particles).
: Then collect the layers and run on western blot to see and compare.
: take I : 17300 rpm at 4C, x 90 min --> no separation
: take II: 30000 rpm at 4C, x 90 min --> no separation
: take III: 30000 rpm at 4C, x 3 h ---> still no separation
: the sucrose pads i used are: 20% (upper), 35% (middle); 50% (bottom); let
: sit at 4C overnight before loading samples and ran the program.
rate of your virion, you can have an educated guess about the conditions you
need.
the
).
【在 a*****s 的大作中提到】
![](/moin_static193/solenoid/img/up.png)
: Need to separate some virions with Sucrose ultracentrifugation (PI said we
: don't need to do CsCl :( - that would have been easier to search for
: literature if so). Anyway, I did some trial runs. My goal is to separate the
: samples to several layers (if there is different shape of viral particles).
: Then collect the layers and run on western blot to see and compare.
: take I : 17300 rpm at 4C, x 90 min --> no separation
: take II: 30000 rpm at 4C, x 90 min --> no separation
: take III: 30000 rpm at 4C, x 3 h ---> still no separation
: the sucrose pads i used are: 20% (upper), 35% (middle); 50% (bottom); let
: sit at 4C overnight before loading samples and ran the program.
m*3
6 楼
父母是中国公民,从北京出发,不知道在加拿大转机是否也需要加拿大的签证?
谢谢
谢谢
E*h
7 楼
中国公民乘坐加航航班经加国到达美国或者离开美国,只要有有效中国护照,有效美国
签证,确认的机票,不需要加国签证。
签证,确认的机票,不需要加国签证。
y*g
8 楼
我母亲从芝加哥飞往北京,中途在多伦多,温哥华转两次,买的全是air Canada (AC)
的机票,请问需要加国签证吗?
的机票,请问需要加国签证吗?
c*e
14 楼
http://www.aircanada.com/en/travelinfo/airport/yyz_intl_us.html
在加航网站上可以查
【在 m******3 的大作中提到】![](/moin_static193/solenoid/img/up.png)
: 父母是中国公民,从北京出发,不知道在加拿大转机是否也需要加拿大的签证?
: 谢谢
在加航网站上可以查
【在 m******3 的大作中提到】
![](/moin_static193/solenoid/img/up.png)
: 父母是中国公民,从北京出发,不知道在加拿大转机是否也需要加拿大的签证?
: 谢谢
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