h*k
2 楼
流式经常堵,使用过bleach, trypsin处理还是没解决问题,打算用Dnase尝试下。问下
班上有人用过这个吗?浓度该用多少比较合适?100ug/mL?
班上有人用过这个吗?浓度该用多少比较合适?100ug/mL?
c*r
4 楼
可以试试。不过可能用处不大。有些cell lines就是臭名昭著的团结一致,死后打不成
single cell suspension。没办法,只能多准备的cell,多pipette up and down,然
后过个filter,再上flow
single cell suspension。没办法,只能多准备的cell,多pipette up and down,然
后过个filter,再上flow
q*g
6 楼
有人有过这种情况吗?可不可以要求改?
s*n
7 楼
Kiss of Death
Try to withdraw one.
Try to withdraw one.
q*g
8 楼
有这么厉害?怎么withdraw?
e*1
9 楼
遇到同样的问题,求建议!明天联系PO.
g*2
10 楼
也倒未必。原则肯定是避免俩R01进入同一个section. 但人算不如天算情况总有发生。
认识一朋友,不幸俩proposal进入同一个了,然后一个被给了negative comment, 说
obviously the PI has no experience. 不过另外一个proposal被fund了。
如果是一个R21,一个R01那没事。
认识一朋友,不幸俩proposal进入同一个了,然后一个被给了negative comment, 说
obviously the PI has no experience. 不过另外一个proposal被fund了。
如果是一个R21,一个R01那没事。
e*1
11 楼
今天联系NIH了,沟通了老半天,最后同意帮忙试试能不能转到另一个study section,
不过不能保证,要做好不能转的准备。又问了熟悉的PO,也不建议在同一个section,原
因是自己compete,而且一般情况别人都不希望你拿两个,尤其新PI有两个显得太
ambitious了,万一落到同一个reviewer手里那就更不好了。
不过不能保证,要做好不能转的准备。又问了熟悉的PO,也不建议在同一个section,原
因是自己compete,而且一般情况别人都不希望你拿两个,尤其新PI有两个显得太
ambitious了,万一落到同一个reviewer手里那就更不好了。
q*g
17 楼
两个PROPOSALS被分到同一个NIH STUDY SECTION,怎么办?
q*g
20 楼
有人有过这种情况吗?可不可以要求改?
s*n
21 楼
Kiss of Death
Try to withdraw one.
Try to withdraw one.
q*g
22 楼
有这么厉害?怎么withdraw?
e*1
23 楼
遇到同样的问题,求建议!明天联系PO.
g*2
24 楼
也倒未必。原则肯定是避免俩R01进入同一个section. 但人算不如天算情况总有发生。
认识一朋友,不幸俩proposal进入同一个了,然后一个被给了negative comment, 说
obviously the PI has no experience. 不过另外一个proposal被fund了。
如果是一个R21,一个R01那没事。
认识一朋友,不幸俩proposal进入同一个了,然后一个被给了negative comment, 说
obviously the PI has no experience. 不过另外一个proposal被fund了。
如果是一个R21,一个R01那没事。
e*1
25 楼
今天联系NIH了,沟通了老半天,最后同意帮忙试试能不能转到另一个study section,
不过不能保证,要做好不能转的准备。又问了熟悉的PO,也不建议在同一个section,原
因是自己compete,而且一般情况别人都不希望你拿两个,尤其新PI有两个显得太
ambitious了,万一落到同一个reviewer手里那就更不好了。
不过不能保证,要做好不能转的准备。又问了熟悉的PO,也不建议在同一个section,原
因是自己compete,而且一般情况别人都不希望你拿两个,尤其新PI有两个显得太
ambitious了,万一落到同一个reviewer手里那就更不好了。
s*c
32 楼
That is not true. I know 2 people getting 2 R01 in the same cycle from NCI.
And we are in a very small field. Their proposals are in different study
section though.
think
【在 A******y 的大作中提到】![](/moin_static193/solenoid/img/up.png)
: No, NIH currently is unlikely to fund two grants from the same PI. I think
: it is actually published in NIH funding priority because my PO told me to
: read it.
And we are in a very small field. Their proposals are in different study
section though.
think
【在 A******y 的大作中提到】
![](/moin_static193/solenoid/img/up.png)
: No, NIH currently is unlikely to fund two grants from the same PI. I think
: it is actually published in NIH funding priority because my PO told me to
: read it.
l*e
33 楼
Nothing is impossible.
My previous PhD Co-advisor just got two R01s from the same study section in
the same cycle. She got the notification from PO today.
She is the contacting PI for one proposal and one of the two MPIs for the ot
her proposal.
NCI.
【在 s*****c 的大作中提到】![](/moin_static193/solenoid/img/up.png)
: That is not true. I know 2 people getting 2 R01 in the same cycle from NCI.
: And we are in a very small field. Their proposals are in different study
: section though.
:
: think
My previous PhD Co-advisor just got two R01s from the same study section in
the same cycle. She got the notification from PO today.
She is the contacting PI for one proposal and one of the two MPIs for the ot
her proposal.
NCI.
【在 s*****c 的大作中提到】
![](/moin_static193/solenoid/img/up.png)
: That is not true. I know 2 people getting 2 R01 in the same cycle from NCI.
: And we are in a very small field. Their proposals are in different study
: section though.
:
: think
w*0
34 楼
case by case吧,看PO怎么说,我的情况,目前一个已有分数,在等budget,另一个明
年2月出分数,都在同一个PO手里,PO说如果两个分数都进线了可能会挑那个分数好的
fund,因为他们倾向于支持更多的PI。我估计没被fund的那个还是可以resubmit的吧。
年2月出分数,都在同一个PO手里,PO说如果两个分数都进线了可能会挑那个分数好的
fund,因为他们倾向于支持更多的PI。我估计没被fund的那个还是可以resubmit的吧。
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