求助：从EMSA实验计算出来的解离常数kd是不是就是表观解离常数kapp? - 未名空间MITBBS历史存档

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求助：从EMSA实验计算出来的解离常数kd是不是就是表观解离常数kapp?

求助：从EMSA实验计算出来的解离常数kd是不是就是表观解离常数kapp?# Biology - 生物学

d*e2011-12-21 08:12

1 楼

通过gel shift assay (EMSA)计算了蛋白跟一系列底物的结合常数kd.
但是审稿人说：EMSA measurements are non-equilibrium and do not yield Kd
values.
请教应该如何回应？
有些文献用表观解离常数kapp来替代kd，这个是否正确？
谢谢。

b*y2011-12-21 08:12

2 楼

I agree with the referee. But does the absolute Kd matter in your case or
the relative Kd? For example, you want to demonstrate wt and mutant proteins
have a big difference in binding the same ligand. In either case, to a
reasonable referee, you might be OK if using Kapp instead of Kd in the paper
and also acknowledge your agreement with his/her opinion in the rebuttal
letter. Good luck!

d*e2011-12-21 08:12

3 楼

谢谢回复。
我只是想比较同一蛋白对不同底物的结合能力。Kapp应该可以说明问题。

proteins
paper

【在 b******y 的大作中提到】

: I agree with the referee. But does the absolute Kd matter in your case or
: the relative Kd? For example, you want to demonstrate wt and mutant proteins
: have a big difference in binding the same ligand. In either case, to a
: reasonable referee, you might be OK if using Kapp instead of Kd in the paper
: and also acknowledge your agreement with his/her opinion in the rebuttal
: letter. Good luck!

b*y2011-12-21 08:12

4 楼

I agree.