请教phage transduction相关问题 - 未名空间MITBBS历史存档

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请教phage transduction相关问题

请教phage transduction相关问题# Biology - 生物学

A*X2012-01-20 08:01

1 楼

有两篇还没写完的文章，有点想往申请的CV上写
但是不知道这方面有没有什么不成文的规定？
比如，列上了in preparation的文章会不会显得不professional
或者给人一种想混水摸鱼的感觉?
申请的领域是MSE/ME
多谢！

T*e2012-01-20 08:01

2 楼

【以下文字转载自 Complain 讨论区】
发信人: mitbbs (未名空间), 信区: Complain
标题: Re: 投诉：请解封我的主ID
发信站: BBS 未名空间站 (Thu May 6 04:19:58 2010, 美东)
请问主ID？

p*n2012-01-20 08:01

3 楼

我刚开始做金葡菌的致病因子，要用phage从一种strain上载入一个mutant DNA片段导
入另一个strain。用的phage是transducing phage，据提供者说high frequency of
lysogenization。我用plaques提取lytic phage，然后和recipient混合，incubate 20
分钟以后立即稀释、涂板，结果没有一个带resistant marker的colony长出来。
我的实验方法可能会出什么问题？
这种phage是不是主要进行specialized transduction？收集lysogen然后transduce是
不是可行的方法？
谢谢各位。

f*72012-01-20 08:01

4 楼

一两片无所谓，太多就不好了。

【在 A********X 的大作中提到】

: 有两篇还没写完的文章，有点想往申请的CV上写
: 但是不知道这方面有没有什么不成文的规定？
: 比如，列上了in preparation的文章会不会显得不professional
: 或者给人一种想混水摸鱼的感觉?
: 申请的领域是MSE/ME
: 多谢！

A*22012-01-20 08:01

5 楼

If I understood your question correctly, you have inserted a Ab resistance
gene (linked to the mutation) into the mutant chr and were trying to move it
into wild type strain. Why not just make a phage lysate of your mutant
strain and then transduce the wild type strain.

s*x2012-01-20 08:01

6 楼

If under review put it in. If "in preparation", don't feel it helps but it
won't hurt you either.

p*n2012-01-20 08:01

7 楼

yes, you got my question.
The mutant comes with an Ab-resistant marker.
I am culture phage with mutant strain, and then collect the phage from
plaques by homogenizing the agar chunks. However, these phage do not produce
Ab resistant colonies on the Ab-selective plates.
I am wondering if only the lysogens can carry the mutant gene with Ab
resistance marker.
Thank you for replying me.

it

【在 A********2 的大作中提到】

: If I understood your question correctly, you have inserted a Ab resistance
: gene (linked to the mutation) into the mutant chr and were trying to move it
: into wild type strain. Why not just make a phage lysate of your mutant
: strain and then transduce the wild type strain.

k*i2012-01-20 08:01

8 楼

放干货吧
别拖泥带水的，其实没必要

【在 A********X 的大作中提到】

A*22012-01-20 08:01

9 楼

My suggestion is:
1. Test if your recipient strain is phage sensitive
2. Determine how many PFU per ml in your lysate by phage titration assay and
use the appropriate amount of lysate in the transduction.

d*n2012-01-20 08:01

10 楼

如果pub不多可以考虑放

【在 A********X 的大作中提到】

A*X2012-01-20 08:01

11 楼

pub数量倒是还行，一作文章数量其实应该也还过得去
但问题是publication list列完了才发现最近好像和人合作太多了，
最新的(自然而然的也就是列最前面的)五六篇全是co-authored文章
于是开始担心sc会不会就扫了一下publication list开头，
然后断定这人只会蹭文章。。。

【在 d********n 的大作中提到】

: 如果pub不多可以考虑放

m*a2012-01-20 08:01

12 楼

"HOWEVER, under NO circumstances should you include publications that are "
in preparation" or "to be submitted." These are always the source of jokes
and negative comments in committee meetings. Any of us can think of some
dozen or so publications that are in preparation. "
---INFORMAL ADVICE ON APPLYING FOR ENGINEERING FACULTY POSITIONS
--- Ken Goodson

【在 f*******7 的大作中提到】

: 一两片无所谓，太多就不好了。

m*12012-01-20 08:01

13 楼

One time I saw a faculty applicant says in the research statement that his/
her total pending funds are several million dollars. After a few months, I
am curous to check and find none was funded.

l*s2012-01-20 08:01

14 楼

有一次我们收到一个日本人在美国做Faculty的申请，说他有一个在日本的Grant，有好
几千万。后来发现单位是日元

【在 m****1 的大作中提到】

: One time I saw a faculty applicant says in the research statement that his/
: her total pending funds are several million dollars. After a few months, I
: am curous to check and find none was funded.

C*g2012-01-20 08:01

15 楼

don't

【在 A********X 的大作中提到】

s*n2012-01-20 08:01

16 楼

我怎么觉得没问题？如果你文章数不够多，放了也是白放，不放也没人因为你诚实就给
你机会。相反，如果你文章数够多，也没人会因为这个觉得你凑数，如果是很有趣的文
章，放上去让人看看你手里还有些东西在做着也不错。
我昨天让career counsel给我改CV她还专门问我除了我列出来的两篇，有没有别的文章
in preparation。