m*r
2 楼
用手机拨号，打出的电话都是google voice的号码，而不是手机自己的号码
接听打到手机号码的电话也要按个1才能听
找不到怎么设置
谢谢！
接听打到手机号码的电话也要按个1才能听
找不到怎么设置
谢谢！
y*n
3 楼
用TIRF or fluorescence microscopy来研究tubulin dynamic in vitro regulated by
other tip proteins.
But now I met a lot of problems:
1. Is labeled tubulin and tubulin easy to lost their activity in vitro?
2. What conditions is better for their polymerization?
以前看到过很好的GMPCPP seeds， 但之后再也重复不出来了。同一批labeled的
tubulin蛋白好象都形成一些很亮的点，应该是蛋白凝聚了或变性了。但新labeled 的
tubulin 也不行。有人有什么建议吗？
other tip proteins.
But now I met a lot of problems:
1. Is labeled tubulin and tubulin easy to lost their activity in vitro?
2. What conditions is better for their polymerization?
以前看到过很好的GMPCPP seeds， 但之后再也重复不出来了。同一批labeled的
tubulin蛋白好象都形成一些很亮的点，应该是蛋白凝聚了或变性了。但新labeled 的
tubulin 也不行。有人有什么建议吗？
n*y
4 楼
狗狗不傻，为啥要做那么好
m*r
5 楼
设了screen off，接听电话不用按 1了
可是打出的号码，和发出的txt，都还是从gv号码出去的
以前和skype可以互通txt，现在能收到但是发布出去了。。。
可是打出的号码，和发出的txt，都还是从gv号码出去的
以前和skype可以互通txt，现在能收到但是发布出去了。。。
l*1
6 楼
GTP→GDP already
please go to new book
"New Insights into the Mechanisms
of Cytomotive Actin and Tubulin
Filaments" 2011 Elsevier Inc.
by Christopher H.S. Aylett, Jan Lo¨we, and Linda A. Amos
full text link:
//www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/AmosReview2011.pdf
its pp20
On phosphate release, Wang and Nogales proposed that the contact
between T7 and the nucleotide of the adjacent subunit within the E-site
interface becomes disrupted, leaving contacts only on the side of the
subunit–subunit interface further from the nucleotide, therefore placing it
in a fully curved-like state. The effect of nucleotide hydrolysis would
therefore be to disrupt the subunit–subunit interface and favor a less stable
form of the tubulin protofilament.
ignored.
by
的
【在 y******n 的大作中提到】
: 用TIRF or fluorescence microscopy来研究tubulin dynamic in vitro regulated by
: other tip proteins.
: But now I met a lot of problems:
: 1. Is labeled tubulin and tubulin easy to lost their activity in vitro?
: 2. What conditions is better for their polymerization?
: 以前看到过很好的GMPCPP seeds， 但之后再也重复不出来了。同一批labeled的
: tubulin蛋白好象都形成一些很亮的点，应该是蛋白凝聚了或变性了。但新labeled 的
: tubulin 也不行。有人有什么建议吗？
please go to new book
"New Insights into the Mechanisms
of Cytomotive Actin and Tubulin
Filaments" 2011 Elsevier Inc.
by Christopher H.S. Aylett, Jan Lo¨we, and Linda A. Amos
full text link:
//www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/AmosReview2011.pdf
its pp20
On phosphate release, Wang and Nogales proposed that the contact
between T7 and the nucleotide of the adjacent subunit within the E-site
interface becomes disrupted, leaving contacts only on the side of the
subunit–subunit interface further from the nucleotide, therefore placing it
in a fully curved-like state. The effect of nucleotide hydrolysis would
therefore be to disrupt the subunit–subunit interface and favor a less stable
form of the tubulin protofilament.
ignored.
by
的
【在 y******n 的大作中提到】
: 用TIRF or fluorescence microscopy来研究tubulin dynamic in vitro regulated by
: other tip proteins.
: But now I met a lot of problems:
: 1. Is labeled tubulin and tubulin easy to lost their activity in vitro?
: 2. What conditions is better for their polymerization?
: 以前看到过很好的GMPCPP seeds， 但之后再也重复不出来了。同一批labeled的
: tubulin蛋白好象都形成一些很亮的点，应该是蛋白凝聚了或变性了。但新labeled 的
: tubulin 也不行。有人有什么建议吗？
p*9
7 楼
故意恶心水果的，Youtube有啥好看的？我从来不用
【在 l******1 的大作中提到】
: YouTube app这么久了也更新的好多次还是问题一堆
【在 l******1 的大作中提到】
: YouTube app这么久了也更新的好多次还是问题一堆
w*3
8 楼
不是可以弄成同一个号码吗……
l*1
9 楼
Continue:
please look a lo below figure
cited from upper floor that E-Book.
【在 l**********1 的大作中提到】
: GTP→GDP already
: please go to new book
: "New Insights into the Mechanisms
: of Cytomotive Actin and Tubulin
: Filaments" 2011 Elsevier Inc.
: by Christopher H.S. Aylett, Jan Lo¨we, and Linda A. Amos
: full text link:
: //www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/AmosReview2011.pdf
: its pp20
: On phosphate release, Wang and Nogales proposed that the contact
please look a lo below figure
cited from upper floor that E-Book.
【在 l**********1 的大作中提到】
: GTP→GDP already
: please go to new book
: "New Insights into the Mechanisms
: of Cytomotive Actin and Tubulin
: Filaments" 2011 Elsevier Inc.
: by Christopher H.S. Aylett, Jan Lo¨we, and Linda A. Amos
: full text link:
: //www2.mrc-lmb.cam.ac.uk/groups/JYL/PDF/AmosReview2011.pdf
: its pp20
: On phosphate release, Wang and Nogales proposed that the contact
u*a
10 楼
最近这个版本比以前还是好多了...没觉得有太大问题.
【在 l******1 的大作中提到】
: YouTube app这么久了也更新的好多次还是问题一堆
【在 l******1 的大作中提到】
: YouTube app这么久了也更新的好多次还是问题一堆
m*r
11 楼
只是装了一个gv，没想到它integrated了。。。
s*n
12 楼
Hope these will help:
http://mitchison.med.harvard.edu/protocols/poly.html
http://hymanlab.mpi-cbg.de/hyman_lab/methods/tubulin/tubulin_pr
http://www.mpi-cbg.de/nc/research/research-groups/joe-howard/pu (look for "Microtubule dynamics reconstituted in vitro and imaged by single-molecule fluorescence microscopy")
BTW: what TIPS proteins?
by
的
【在 y******n 的大作中提到】
: 用TIRF or fluorescence microscopy来研究tubulin dynamic in vitro regulated by
: other tip proteins.
: But now I met a lot of problems:
: 1. Is labeled tubulin and tubulin easy to lost their activity in vitro?
: 2. What conditions is better for their polymerization?
: 以前看到过很好的GMPCPP seeds， 但之后再也重复不出来了。同一批labeled的
: tubulin蛋白好象都形成一些很亮的点，应该是蛋白凝聚了或变性了。但新labeled 的
: tubulin 也不行。有人有什么建议吗？
http://mitchison.med.harvard.edu/protocols/poly.html
http://hymanlab.mpi-cbg.de/hyman_lab/methods/tubulin/tubulin_pr
http://www.mpi-cbg.de/nc/research/research-groups/joe-howard/pu (look for "Microtubule dynamics reconstituted in vitro and imaged by single-molecule fluorescence microscopy")
BTW: what TIPS proteins?
by
的
【在 y******n 的大作中提到】
: 用TIRF or fluorescence microscopy来研究tubulin dynamic in vitro regulated by
: other tip proteins.
: But now I met a lot of problems:
: 1. Is labeled tubulin and tubulin easy to lost their activity in vitro?
: 2. What conditions is better for their polymerization?
: 以前看到过很好的GMPCPP seeds， 但之后再也重复不出来了。同一批labeled的
: tubulin蛋白好象都形成一些很亮的点，应该是蛋白凝聚了或变性了。但新labeled 的
: tubulin 也不行。有人有什么建议吗？
w*2
13 楼
YouTube 单拿出来应该对google不差，youtube已经算垄断了，在iphone上单出来可以
放广告，对google是好事。
版本现在的还不错了。
放广告，对google是好事。
版本现在的还不错了。
a*n
14 楼
友情提醒GV打电话要算分钟
不走data
我把这个破玩意儿善了
不走data
我把这个破玩意儿善了
l*1
15 楼
Are you sure you can use only fluorescence microscopy来研究tubulin dynamics
in vitro regulated by other tip protein?
http://www.mitbbs.com/article_t1/Biology/31629913_0_2.html
27th floor
If not,
please just go to
//cryoem.berkeley.edu/microtubules.html
//cryoem.berkeley.edu/cryoem
//cryoem.berkeley.edu/microscopes
more just click all icons you can open that web sites.
//mcb.berkeley.edu/index.php?option=com_mcbfaculty&name=nogalese
then rethink about which kind of instrument will be needed?
by
的
【在 y******n 的大作中提到】
: 用TIRF or fluorescence microscopy来研究tubulin dynamic in vitro regulated by
: other tip proteins.
: But now I met a lot of problems:
: 1. Is labeled tubulin and tubulin easy to lost their activity in vitro?
: 2. What conditions is better for their polymerization?
: 以前看到过很好的GMPCPP seeds， 但之后再也重复不出来了。同一批labeled的
: tubulin蛋白好象都形成一些很亮的点，应该是蛋白凝聚了或变性了。但新labeled 的
: tubulin 也不行。有人有什么建议吗？
in vitro regulated by other tip protein?
http://www.mitbbs.com/article_t1/Biology/31629913_0_2.html
27th floor
If not,
please just go to
//cryoem.berkeley.edu/microtubules.html
//cryoem.berkeley.edu/cryoem
//cryoem.berkeley.edu/microscopes
more just click all icons you can open that web sites.
//mcb.berkeley.edu/index.php?option=com_mcbfaculty&name=nogalese
then rethink about which kind of instrument will be needed?
by
的
【在 y******n 的大作中提到】
: 用TIRF or fluorescence microscopy来研究tubulin dynamic in vitro regulated by
: other tip proteins.
: But now I met a lot of problems:
: 1. Is labeled tubulin and tubulin easy to lost their activity in vitro?
: 2. What conditions is better for their polymerization?
: 以前看到过很好的GMPCPP seeds， 但之后再也重复不出来了。同一批labeled的
: tubulin蛋白好象都形成一些很亮的点，应该是蛋白凝聚了或变性了。但新labeled 的
: tubulin 也不行。有人有什么建议吗？
w*2
16 楼
可以听相声
【在 p******9 的大作中提到】
: 故意恶心水果的，Youtube有啥好看的？我从来不用
【在 p******9 的大作中提到】
: 故意恶心水果的，Youtube有啥好看的？我从来不用
m*r
17 楼
这个还好，只要别人看到是我的号码
【在 a******n 的大作中提到】
: 友情提醒GV打电话要算分钟
: 不走data
: 我把这个破玩意儿善了
【在 a******n 的大作中提到】
: 友情提醒GV打电话要算分钟
: 不走data
: 我把这个破玩意儿善了
s*n
18 楼
I think the in vitro tubulin dynamics experiments using TIRF microscopy will
be able to say whether the presence of tip protein(s) affects the tubulin
rescue and shrink rates. A complete understanding of the underlying
molecular mechanism may require structural studies using other methods
including EM.
dynamics
【在 l**********1 的大作中提到】
: Are you sure you can use only fluorescence microscopy来研究tubulin dynamics
: in vitro regulated by other tip protein?
: http://www.mitbbs.com/article_t1/Biology/31629913_0_2.html
: 27th floor
: If not,
: please just go to
: //cryoem.berkeley.edu/microtubules.html
: //cryoem.berkeley.edu/cryoem
: //cryoem.berkeley.edu/microscopes
: more just click all icons you can open that web sites.
be able to say whether the presence of tip protein(s) affects the tubulin
rescue and shrink rates. A complete understanding of the underlying
molecular mechanism may require structural studies using other methods
including EM.
dynamics
【在 l**********1 的大作中提到】
: Are you sure you can use only fluorescence microscopy来研究tubulin dynamics
: in vitro regulated by other tip protein?
: http://www.mitbbs.com/article_t1/Biology/31629913_0_2.html
: 27th floor
: If not,
: please just go to
: //cryoem.berkeley.edu/microtubules.html
: //cryoem.berkeley.edu/cryoem
: //cryoem.berkeley.edu/microscopes
: more just click all icons you can open that web sites.
k*g
19 楼
foxtube 可以cache 下面，background 播放。
新版本被youtube 要求disable。
从来不用youtube app
【在 w********2 的大作中提到】
: 可以听相声
新版本被youtube 要求disable。
从来不用youtube app
【在 w********2 的大作中提到】
: 可以听相声
a*n
20 楼
你用电话直接拨
别人看到的就是你的号码
【在 m***r 的大作中提到】
: 这个还好，只要别人看到是我的号码
别人看到的就是你的号码
【在 m***r 的大作中提到】
: 这个还好，只要别人看到是我的号码
l*1
21 楼
Exactly your opinion is right.
plus even without CryoEM below group still can do simulation with modeling for their most possbile
mechanism discovery
please refer the figure G its scale bar 200 nm it is below florescent microscopy resolution limits,
so they cannot resolve the fusion of the individual FIP3-endosomes.
cited from
//www.ncbi.nlm.nih.gov/pubmed/21486954
and
//www.ncbi.nlm.nih.gov/pubmed/16818889
will
【在 s****n 的大作中提到】
: I think the in vitro tubulin dynamics experiments using TIRF microscopy will
: be able to say whether the presence of tip protein(s) affects the tubulin
: rescue and shrink rates. A complete understanding of the underlying
: molecular mechanism may require structural studies using other methods
: including EM.
:
: dynamics
plus even without CryoEM below group still can do simulation with modeling for their most possbile
mechanism discovery
please refer the figure G its scale bar 200 nm it is below florescent microscopy resolution limits,
so they cannot resolve the fusion of the individual FIP3-endosomes.
cited from
//www.ncbi.nlm.nih.gov/pubmed/21486954
and
//www.ncbi.nlm.nih.gov/pubmed/16818889
will
【在 s****n 的大作中提到】
: I think the in vitro tubulin dynamics experiments using TIRF microscopy will
: be able to say whether the presence of tip protein(s) affects the tubulin
: rescue and shrink rates. A complete understanding of the underlying
: molecular mechanism may require structural studies using other methods
: including EM.
:
: dynamics
p*9
22 楼
油库，sohu tv都有吧，还可以离线
【在 w********2 的大作中提到】
: 可以听相声
【在 w********2 的大作中提到】
: 可以听相声
m*r
23 楼
不是，看到的是我GV的号码。txt也是GV的号码。
我没有把mobile号码过继给GV，用的是option 2，难道这样还是一切走GV？
【在 a******n 的大作中提到】
: 你用电话直接拨
: 别人看到的就是你的号码
我没有把mobile号码过继给GV，用的是option 2，难道这样还是一切走GV？
【在 a******n 的大作中提到】
: 你用电话直接拨
: 别人看到的就是你的号码
S*s
24 楼
这很正常，你去狗子化，狗子也能用垃圾恶心你。
互利互惠才是正确的
互利互惠才是正确的
l*e
25 楼
在桌面上放个gv的小插件，然后在call 那里第三个选项那里选择do not use或者
international only
international only
m*r
26 楼
是按住gv拖到桌面上那种插件吗？我在里面找不到call的选项。一进去默认就是inbox
，菜单里也没有。。。
还有短信怎么改默认成电话的？别人看我短信也是gv号码，还没试是否支持中文
谢谢老饭！
【在 l*****e 的大作中提到】
: 在桌面上放个gv的小插件，然后在call 那里第三个选项那里选择do not use或者
: international only
，菜单里也没有。。。
还有短信怎么改默认成电话的？别人看我短信也是gv号码，还没试是否支持中文
谢谢老饭！
【在 l*****e 的大作中提到】
: 在桌面上放个gv的小插件，然后在call 那里第三个选项那里选择do not use或者
: international only
相关阅读
跪求stem cell, cardiovascular regeneration方向审稿中科大少年班52名神童报到诚招博后,癌症表观遗传以及非编码RNA马上要走人了，LB要我把我的技术全部教会给实验室其他人，怎么办。生物类企业的博士后怎么样，有过来人么？请教有没有一种研究名字叫case-case control###此帖已应当事人要求删除###Dior, LV, Rolex, Benz. Etc. luxury goods.questions about viral RNA detection苦乐何所依问一个leukemia的分类新杂志 J Biol Methods， 请捧场你们的呕像是无耻小人！天然的不同蛋白质之间的共价连接？RNA-seq input的问题招聘博士后邵锋的弱点信息: 6600美元在Udacity学佐治亚理工学院的CS Master骨科医生为啥也能得诺贝尔奖？Molecular cell 如果文章送审了会通知吗