m*M
4 楼
I specially tested overnight e coli cultures harboring plasmid with and
without antibotics, and turned out that I even got more plasmids than those
with antibotics. Try to isolate them (one or two if you don't want to waste
your miniprep column), and tell us your results.
without antibotics, and turned out that I even got more plasmids than those
with antibotics. Try to isolate them (one or two if you don't want to waste
your miniprep column), and tell us your results.
g*j
5 楼
如果等会儿有时间就提一下,不过以前没提过这个质粒,所以产率没法儿比较
those
waste
【在 m****M 的大作中提到】![](/moin_static193/solenoid/img/up.png)
: I specially tested overnight e coli cultures harboring plasmid with and
: without antibotics, and turned out that I even got more plasmids than those
: with antibotics. Try to isolate them (one or two if you don't want to waste
: your miniprep column), and tell us your results.
those
waste
【在 m****M 的大作中提到】
![](/moin_static193/solenoid/img/up.png)
: I specially tested overnight e coli cultures harboring plasmid with and
: without antibotics, and turned out that I even got more plasmids than those
: with antibotics. Try to isolate them (one or two if you don't want to waste
: your miniprep column), and tell us your results.
s*9
6 楼
还是不要提了,重新接种,重新摇,别忘了加抗生素。
g*5
7 楼
my labmate asked plasmid from me and put it directly in LB, and she tried 3
times, no cloudy and asked me why....
times, no cloudy and asked me why....
M*n
11 楼
start over.
it will save you time.
it will save you time.
l*2
15 楼
我干过,没事。。。
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