阴离子交换色谱,用 TrisHCl buffer pH 5.5 洗, elute pH 9. - 未名空间MITBBS历史存档

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阴离子交换色谱,用 TrisHCl buffer pH 5.5 洗, elute pH 9.

阴离子交换色谱,用 TrisHCl buffer pH 5.5 洗, elute pH 9.# Biology - 生物学

b*e2012-06-05 07:06

1 楼

You are a given a very large line delimited file. With a phone number on
each line. How would you remove all duplicates?
1. Can you remove duplicates in less than O (n)?
2. How about the file is too large, out of memory limit?

j*a2012-06-05 07:06

2 楼

阴离子交换色谱 Q Sepharose Fast Flow (GE Healthcare Life Sciences)pH
stability Working Range 2-12 .用1 M NaOH 洗,用纯水洗到pH 7, 换TrisHCl
buffer pH 5.5 平衡, elute pH 变成9.洗了一天还是pH 9. 有人遇到过吗？

g*y2012-06-05 07:06

3 楼

第一个咋做到第二个可以用bit array吧

【在 b********e 的大作中提到】

: You are a given a very large line delimited file. With a phone number on
: each line. How would you remove all duplicates?
: 1. Can you remove duplicates in less than O (n)?
: 2. How about the file is too large, out of memory limit?

s*n2012-06-05 07:06

4 楼

You column is stable between 2-12. However, you washed it by 1 M NaOH?!
And why did you use Tris-HCl for pH 5.5? Tris-HCl also works between 7-9.
Did you measure the pH offline or online? If online, did you calibrate the
pH sensor?

b*e2012-06-05 07:06

5 楼

I don't know how to do it:(

【在 g*******y 的大作中提到】

: 第一个咋做到 : 第二个可以用bit array吧

j*a2012-06-05 07:06

6 楼

Good point. I need to switch buffer system.

j*a2012-06-05 07:06

7 楼

For the first question, I guess the interviewer want to get a firm answer
of "no, it is impossible!". :)

【在 b********e 的大作中提到】

: I don't know how to do it:(