Re; Cosimo2014 (柯希莫) 两重串联Mass 乃至傅立叶变换 三重串联Mass 和传统Mass的分析蛋白质的区别是 后者只有分子量的定性 和相对稳定同位素标示后的比如 SILAC Mass的定量 而前者 可以测 wild pH (NH4)2 SO4 溶液 pH 6.9 中的蛋白 二级结构 数据 乃至 dimer trimer etc. 三级结构数据 please go to below link: //www.findnano.fi/fi/NanoCrmEquipment:UserView/id/1431 only some titles are Finnish for example: Tärkeimmät spesifikaatiot: (NB: Key Specifications by //translate.google.fr/?hl=fr/ from Finnish to English bla bla bla...) then almost rest is english: Multistage MS (MS3 guaranteed): LHRH MS/MS (collision cell) -> MS/MS/MS ( Infinity Cell™) or you had interests: just check one paper about Top-down Mass Laitaoja M et al. and Jänis J. (2012) Top-down mass spectrometry reveals new sequence variants of the major bovine seminal plasma protein PDC-109. J Mass Spectrom. 47: 853-9. PubMed link: //www.ncbi.nlm.nih.gov/pubmed/22791252 or //www.joensuu.fi/kemia/research/protein/research.ms1.html or Native Mass //www.joensuu.fi/kemia/research/protein/publica.html Good Luck! Ps: matrix just met that paper its last/corresponding author: web link: //www.uef.fi/mass-spectrometry/janne-janis at an international congress this summer. ----- 发信人: KingofMS (你太有才了), 信区: Biology 标 题: Re: [求教] LC-MS/MS 和LC/MS/MS一个意思吗? 发信站: BBS 未名空间站 (Sat Sep 1 01:22:56 2012, 美东) tandem MS can be tandem in time or tandem in space. For example, a single ion trap can do both MS and MS/MS.
【在 l**********1 的大作中提到】 : Re; Cosimo2014 (柯希莫) : 两重串联Mass 乃至傅立叶变换 三重串联Mass 和传统Mass的分析蛋白质的区别是 : 后者只有分子量的定性 和相对稳定同位素标示后的比如 SILAC Mass的定量 : 而前者 可以测 wild pH (NH4)2 SO4 溶液 pH 6.9 中的蛋白 二级结构 数据 乃至 : dimer trimer etc. 三级结构数据 : please go to below link: : //www.findnano.fi/fi/NanoCrmEquipment:UserView/id/1431 : only some titles are Finnish : for example: : Tärkeimmät spesifikaatiot:
l*1
15 楼
matrix 做质谱分析的那1年 你还在吃奶还是高中啊 almost two decade ago?
【在 S*********s 的大作中提到】 : 你贴这么多 你自己看的懂吗?
K*S
16 楼
All were liquid when Wolfgang paul was working on MS.
【在 l**********1 的大作中提到】 : matrix 做质谱分析的那1年 你还在吃奶还是高中啊 almost two decade ago?
l*1
17 楼
当代的生物蛋白质谱学 理科本科毕业 或社会学 经济学 本科毕业的借助wiki and google 都可以看懂>85%的内容 吧 Lamond AI et a. and Mann M. (2012) Advancing cell biology through proteomics in space and time (PROSPECTS). Mol Cell Proteomics. 11: O112.017731. http://www.ncbi.nlm.nih.gov/pubmed/22311636 pp3 left column bottom to right column top Fourier transform algorithm, the Orbitrap ELITE achieves fourfold higher resolution at the same transient length. This enables efficient top-down operation in a chromatographic time scale, as well as ultra-high resolution scans for routine liquid chromatography tandem MS (LC MS/MS) operation. This should dramatically increase the certainty and information content of MS/MS assignments, especially for modified peptides. NB: 上周看不懂 这周说不定就看明白了 哈