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Help me to troubleshoot the digestion of genomic DNA by HindIII
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Help me to troubleshoot the digestion of genomic DNA by HindIII# Biology - 生物学
p*l
1
POSITION: EMI R&D Leader
COMPANY DESCRIPTION
This UK-based company is the world's leading producer of EMI/RFI
shielding materials and related products for the telecommunications,
computer, electronics, networking equipment, aerospace, defense, and
medical equipment industries. It supplies products that shield against
electromagnetic interference (EMI), such as conductive foam, printed
circuit board grounding devices, and gaskets. It also offers antenna
components and thermal reduction devices.
WORKING LOCATION:
• Tianjin, China
RESPONSIBILITIES:
• Researches and develops specific scientific areas in furtherance
of
specific R&D projects.
• Delivery of specialized knowledge and scientific expertise to
further R&D goals.
• Prepares formulations and conducts experiments to high levels of
scientific practice.
• Directs laboratory technicians during experiments.
• Conducts analytical studies using sophisticated laboratory
instruments.
• Prepares comprehensive reports with recommendations for new
product
formulations and procedures.
• Researches scientific literature and vendor product evaluations.
• Works independently and with broad creative latitude.
REQUIREMENTS:
• Experience in a disciplined R&D environment in a commercial or
academic setting.
• Experience as a contributing member of a research team operating
in
an extremely demanding
environment.
• Excellent communication skills, both written and verbal in English.
• Willingness and ability to represent the Company as a lead
technical
expert at customers and
technical organizations.
EDUCATION/EXPERIENCE:
• A Phd degree in a specialized scientific area of study for rubber
(Silicone or EPDM) and composites with function like electrical
conductive or thermal conductive as well as absorb materials, familiar
to manufacturing process like mixing, molding; extruding etc.
• Minimum of 10 years experience in an R&D setting
• Proven contributor in an advanced R&D environment.
• working experience in USA has advance
Contact Person: Ada Wen
Contact No.: 0086-13926439955
MSN: a********[email protected]
Email: a*****[email protected]
========================================================================
TITLE : AVP, Information Management- Bank
LOCATION : Guangzhou
· PART II
· Company Profile
This is the largest, captive, banking and financial services offshoring
organisation in the world. It has more than 32,000 skilled professionals
operating out of 15 Group Service Centres present in five countries in
Asia, including India, China, Malaysia, Philippines and Sri Lanka.
A vital part of the group's global strategy, this company seamlessly
integrates and plays a key role in delivering shareholder value helping
the group remain competitive in the global financial services market.
The company, with two offices in Guangzhou and one office in Foshan
forms part of the group's Global Resourcing network and provides data
processing, customer service facilities and professional services for
the Group's operating entities worldwide including Hong Kong, Macau,
Bermuda, United States, United Kingdom, Canada, Japan and Area office
China. Established in 1996, it has experienced rapid growth in the past
years and grown from 60 staff to more than 4,500.
· PART III
· Principal Responsibilities
· To leverage the information infrastructure to support the
business community.
· To manage the content and effectiveness of the information
infrastructure including the Enterprise Data warehouse, Oracle and SAS
data marts, Reporting and Access Tools and the quality and accuracy of
the content.
· To solve technically complex programming projects in
assigned area.
· To deliver integrated and comprehensible information to
support strategic and tactical information needs of the organization.
· To drive the campaign management process to generate revenue
in the assigned portfolio.
· To design, develops and maintains databases and data marts
as needed for MIS, end-user computing and statistical modeling.
· To manage internal and external resources, processes and
projects to develop key information sources and deliverables to provide
the business with an easy-to-use structure containing the strategic
information necessary to perform complex analysis, modeling and
segmentation.
· To identify and develops new data sources for use throughout
the organization.
· To develop the information required to manage the customer
relationship, employs in the decision engine and leverages information
in all channels of customer contact management process.
· Requirements
· Hold a Bachelor's degree in Computer Science, Mathematics,
Statistics, Economics, Engineering, Business, Social Science and eight
years experience in a computing or information science oriented area.
Master degree is preferable.
· Has excellent people and project management, organizational,
analytical and technical skills.
· Demonstrated proficiency in programming in SAS, Easytrieve,
COBOL, SQL, PEARL, or JAVA.
· Demonstrated ability in managing a multitude of projects and
requests of varying degrees of complexity.
· Has a commitment to provide a high degree of customer
service to both internal and external clients.
· Has the ability to actively listen, interview and apply
information from meetings to complete assigned tasks.
· Has excellent cognitive and mathematical aptitude.
· Has strong interpersonal, presentation, written and verbal
communication skills.
Contact Person: Helen Shi
Contact No.: 0086-13924211922
MSN: h******[email protected]
Email: H*******[email protected]
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m*t
2
老的ipad 2,最近发现充的好慢
可能是啥原因?
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b*r
4
These days, almost make me desperate to death. As a 2-year post-doc,who did
lots of cloning, I fail to do such an easy work. Isolate mouse genomic DNA
by phenol/chloroform,75%ethanol precipitation. Then digest by HindIII
restriction enzyme as follows:
genomic DNA: 2 ug,1ug,and 0.4ug;
HindIII enzyme: 70 units(3.5ul,and different units);
Buffer: 5 ul
add water to 50ul
37 degree for 10 hours. Run gel.Genomic DNA is still a strong band, not
smear at all. I have try HindIII from NEB and Roche companies. Both don't
work. But cut plasmid very well. Any one can tell me how to fix this
problem? It takes me one week to try any ideas I could think out. Please
help me out. My mentor is angry to me again and again. Or else, I think I
have to leave this lab.
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l*s
5
都很慢吧
用这个充手机还挺快的
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s*s
7
你试一下column based的方法抽gDNA吧。我怀疑是不是蛋白没除尽,或者
有phenol残余。

did
not

【在 b******r 的大作中提到】
: These days, almost make me desperate to death. As a 2-year post-doc,who did
: lots of cloning, I fail to do such an easy work. Isolate mouse genomic DNA
: by phenol/chloroform,75%ethanol precipitation. Then digest by HindIII
: restriction enzyme as follows:
: genomic DNA: 2 ug,1ug,and 0.4ug;
: HindIII enzyme: 70 units(3.5ul,and different units);
: Buffer: 5 ul
: add water to 50ul
: 37 degree for 10 hours. Run gel.Genomic DNA is still a strong band, not
: smear at all. I have try HindIII from NEB and Roche companies. Both don't

avatar
m*t
8
ipad mini 充的挺快的

【在 l**s 的大作中提到】
: 都很慢吧
: 用这个充手机还挺快的

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d*0
9
接口全么?
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h*y
10
1. 你抽提的DNA有问题。
2. 试试其他酶,例如EcoRI
by the way, 你用的酶量太大。

did
not

【在 b******r 的大作中提到】
: These days, almost make me desperate to death. As a 2-year post-doc,who did
: lots of cloning, I fail to do such an easy work. Isolate mouse genomic DNA
: by phenol/chloroform,75%ethanol precipitation. Then digest by HindIII
: restriction enzyme as follows:
: genomic DNA: 2 ug,1ug,and 0.4ug;
: HindIII enzyme: 70 units(3.5ul,and different units);
: Buffer: 5 ul
: add water to 50ul
: 37 degree for 10 hours. Run gel.Genomic DNA is still a strong band, not
: smear at all. I have try HindIII from NEB and Roche companies. Both don't

avatar
l*s
11
多长时间?
我的10%- 100%大概要5+小时

【在 m******t 的大作中提到】
: ipad mini 充的挺快的
avatar
g*g
12
这款口碑好像还不错,Price is good
As you've come to expect from Monoprice, we stand behind our products and
offer a full 1 year warranty, which is at least 3-4 times what is offered by
other monitor manufacturers. Additionally, we are so confident of the
quality of these displays that we are guaranteeing these monitor will have
less than 5 dead pixels. If you can count 5 dead pixels anywhere on the
screen, we'll give you a new one. By comparison, the industry standard, even
for industry leaders like Apple and LG, is 10 dead pixels or even more.

【在 g***u 的大作中提到】
: 这个不保证无坏点,5个才换
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g*n
13
有phenol残余的可能性大。用chloroform 再洗一次,再沉淀。
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m*t
14
没统计过,但肯定比ipad 2快。有时,ipad 2插上去15分钟,一点没充进去。

【在 l**s 的大作中提到】
: 多长时间?
: 我的10%- 100%大概要5+小时

avatar
q*j
15
有什么便宜显卡推荐?不打游戏的。

by
even

【在 g****g 的大作中提到】
: 这款口碑好像还不错,Price is good
: As you've come to expect from Monoprice, we stand behind our products and
: offer a full 1 year warranty, which is at least 3-4 times what is offered by
: other monitor manufacturers. Additionally, we are so confident of the
: quality of these displays that we are guaranteeing these monitor will have
: less than 5 dead pixels. If you can count 5 dead pixels anywhere on the
: screen, we'll give you a new one. By comparison, the industry standard, even
: for industry leaders like Apple and LG, is 10 dead pixels or even more.

avatar
b*r
16
Thanks. Can you tell me the name of 'column based' purification of genomic
DNA?

【在 s******s 的大作中提到】
: 你试一下column based的方法抽gDNA吧。我怀疑是不是蛋白没除尽,或者
: 有phenol残余。
:
: did
: not

avatar
m*s
17
你换个10A的充电器。

【在 m******t 的大作中提到】
: 老的ipad 2,最近发现充的好慢
: 可能是啥原因?

avatar
b*r
19
I have done '用chloroform 再洗一次,75% ethanol 再沉淀。',but yield is too
low.
Can I do this:
add NaAc(0.1 volume of genomic DNA), mix,then 2.5 volume of 100% ethanol to
precipitate again? The question is: does a little NaAc still stay in
purified genomic DNA and inhibit digestion of HindIII?

【在 g*****n 的大作中提到】
: 有phenol残余的可能性大。用chloroform 再洗一次,再沉淀。
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t*g
20
这个是没接口的ebay上的韩国货一样,价格不如ebay上的某些韩国的
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g*n
21
The question is: does a little NaAc still stay in
purified genomic DNA and inhibit digestion of HindIII?
Maybe. You can use a large volume (e.g., 0.5 ml) of 70% ethanol to wash the
DNA pellet.
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q*j
22
什么是没借口?

【在 t*****g 的大作中提到】
: 这个是没接口的ebay上的韩国货一样,价格不如ebay上的某些韩国的
avatar
t*g
24
只有DVI, 没有DP, HDMI, 不能连laptop的

【在 q**j 的大作中提到】
: 什么是没借口?
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b*r
25
Thanks a lot
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