Overall, your project sounds interesting. However, you might want to collect
more data or do far more in-depth analysis prior to submitting your story
to top journals. Below are my specific questions:
Where do you obtain this repellent? From plants or from insects?
Can you elaborate on what ecological impacts this repellent has? Can it be
used as insecticide?
If you are sure this repellent is a mixture instead of a pure chemical, can
you identify its chemical components by doing HPLC, per say?
已通过genetic screen筛出了一些为avoidance （针
What genes have you identified by behavior-based genetic screen? Among these
genes, have you found any GPCR receptors or other transmembrane proteins?
If yes, you should focus on characterizing these genes as they are potential
repellent receptors.
通过ablation实验大致确定了哪些神经元是参与
Neuron ablation is one method to identify which neurons participate in
response to this repellent. However,
many drivers are dirty and you should narrow down which subset of neurons
are really recruited by doing more sophisticated genetic labeling
experiments. Moreover,
calcium imaging analysis is required to back up your anatomical mapping
experiments.
GPCR
If you want to try high-profile journals such as Neuron or CNS, identifying
the receptor is the key. ，
转向看哪些G-alpha subunit参与detection。
You would be better off doing calcium imaging if you can.
