s*y
2 楼
We usually cut a piece of tissue and put in RNAlater. This way we can
isolate RNA later.
This time I got some tissue from other lab. They just keep tissue in -80
degree.Has anybody extract RNA from them? How is the quality?
isolate RNA later.
This time I got some tissue from other lab. They just keep tissue in -80
degree.Has anybody extract RNA from them? How is the quality?
C*u
4 楼
It depends on which tissue...
For liver, brain, muscle, white fat and lung, I got very good quality of RNA
from them. But for pancreas, no...
【在 s*********y 的大作中提到】
: We usually cut a piece of tissue and put in RNAlater. This way we can
: isolate RNA later.
: This time I got some tissue from other lab. They just keep tissue in -80
: degree.Has anybody extract RNA from them? How is the quality?
For liver, brain, muscle, white fat and lung, I got very good quality of RNA
from them. But for pancreas, no...
【在 s*********y 的大作中提到】
: We usually cut a piece of tissue and put in RNAlater. This way we can
: isolate RNA later.
: This time I got some tissue from other lab. They just keep tissue in -80
: degree.Has anybody extract RNA from them? How is the quality?
s*y
6 楼
They are lung tissues. But they are not in Trizol or RNAlater.I think they
just freeze the tissue. So I don't want to try.
Pacrease is hard to get good quality RNA, even fresh tissue.
just freeze the tissue. So I don't want to try.
Pacrease is hard to get good quality RNA, even fresh tissue.
s*y
12 楼
I know it's OK to do quick-freeze in liquid nitrogene and leave in -80.
But they just cut the tissue and leave in -80.I guess it's not good.
But they just cut the tissue and leave in -80.I guess it's not good.
a*d
22 楼
楼主不妨问些他/她对tenure&promotion的看法,作为junior,对这方面应该比较关心。
其他能聊的很多,比如学科走向、系的发展方向、能从校方争取到什么资源等等。
其他能聊的很多,比如学科走向、系的发展方向、能从校方争取到什么资源等等。
j*l
27 楼
我也搬个板凳来坐等总结
W*n
28 楼
最重要的是不要把大部分人都得罪了,而是团结与你想法一致的,建立共识。
b*6
30 楼
what to say as a junior faculty member?
a*d
45 楼
楼主不妨问些他/她对tenure&promotion的看法,作为junior,对这方面应该比较关心。
其他能聊的很多,比如学科走向、系的发展方向、能从校方争取到什么资源等等。
其他能聊的很多,比如学科走向、系的发展方向、能从校方争取到什么资源等等。
j*l
50 楼
我也搬个板凳来坐等总结
W*n
51 楼
最重要的是不要把大部分人都得罪了,而是团结与你想法一致的,建立共识。
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