c*b
2 楼
国内一个实验室求yeast,我们完全没有经验,不知道邮寄yeast需要什么怎么准备。好
像没有抗性marker,不知道如何避免bacterial contamination。
多谢指教!
像没有抗性marker,不知道如何避免bacterial contamination。
多谢指教!
w*n
4 楼
Two methods:
1) Streak the strain out on YPD plate (or selective plate), seal the plate,
and mail it in a pressure resistant envelope.
2) Grow the strain up in liquid culture (overnight is enough), put one
droplet (50-100ul) onto a small sterile waterman paper, wrap it with sterile
foil. Mail it in a regular envelope.
【在 c********b 的大作中提到】![](/moin_static193/solenoid/img/up.png)
: 国内一个实验室求yeast,我们完全没有经验,不知道邮寄yeast需要什么怎么准备。好
: 像没有抗性marker,不知道如何避免bacterial contamination。
: 多谢指教!
1) Streak the strain out on YPD plate (or selective plate), seal the plate,
and mail it in a pressure resistant envelope.
2) Grow the strain up in liquid culture (overnight is enough), put one
droplet (50-100ul) onto a small sterile waterman paper, wrap it with sterile
foil. Mail it in a regular envelope.
【在 c********b 的大作中提到】
![](/moin_static193/solenoid/img/up.png)
: 国内一个实验室求yeast,我们完全没有经验,不知道邮寄yeast需要什么怎么准备。好
: 像没有抗性marker,不知道如何避免bacterial contamination。
: 多谢指教!
c*b
6 楼
多谢wenqian!
,
sterile
【在 w*****n 的大作中提到】![](/moin_static193/solenoid/img/up.png)
: Two methods:
: 1) Streak the strain out on YPD plate (or selective plate), seal the plate,
: and mail it in a pressure resistant envelope.
: 2) Grow the strain up in liquid culture (overnight is enough), put one
: droplet (50-100ul) onto a small sterile waterman paper, wrap it with sterile
: foil. Mail it in a regular envelope.
,
sterile
【在 w*****n 的大作中提到】
![](/moin_static193/solenoid/img/up.png)
: Two methods:
: 1) Streak the strain out on YPD plate (or selective plate), seal the plate,
: and mail it in a pressure resistant envelope.
: 2) Grow the strain up in liquid culture (overnight is enough), put one
: droplet (50-100ul) onto a small sterile waterman paper, wrap it with sterile
: foil. Mail it in a regular envelope.
t*h
7 楼
打电话,有票,刚订了,SFO-PVG
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