D*s
2 楼
顶一下，没人有兴趣么？
【在 D***s 的大作中提到】
: 最近比较火的是TALEN和CRISPR-CAS系统，谁能总结一下优缺点么？
: 还有没有别的有潜力的system？
【在 D***s 的大作中提到】
: 最近比较火的是TALEN和CRISPR-CAS系统，谁能总结一下优缺点么？
: 还有没有别的有潜力的system？
l*1
3 楼
cited,
>Both zinc finger nucleases (ZFNs) and TAL effector nucleases (TALENs)
>can be used to mutagenize genomes at specific loci, but
these systems require two different DNA binding proteins
flanking a sequence of interest, each with a C-terminal
FokI nuclease module. As a result these methods have not
been widely adopted by the plant research community.
from
>Belhaj K et al., (2013).
>Plant genome editing made easy: targeted mutagenesis in model and crop
plants using the CRISPR/Cas >system.
>Plant Methods. 9: 39. [Epub ahead of print]
>http://www.ncbi.nlm.nih.gov/pubmed/24112467
or
HTTP double dot //www.plantmethods.com/content/pdf/1746-4811-9-39.pdf
more pls go to,
>Jiang W et al., (2013).
>Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in
Arabidopsis, tobacco, >sorghum and rice.
>Nucleic Acids Res. 41: e188.
>Abstract
>The type II CRISPR/Cas system from Streptococcus pyogenes and its
simplified derivative, the Cas9/single >guide RNA (sgRNA) system, have
emerged as potent new tools for targeted gene knockout in bacteria, >yeast,
fruit fly, zebrafish and human cells. Here, we describe adaptations of these
systems leading to >successful expression of the Cas9/sgRNA system in two
dicot plant species
below ignored
http://www.ncbi.nlm.nih.gov/pubmed/23999092
or
HTTP double dot//nar.oxfordjournals.org/content/early/2013/08/31/nar.gkt780.
full.pdf
>Both zinc finger nucleases (ZFNs) and TAL effector nucleases (TALENs)
>can be used to mutagenize genomes at specific loci, but
these systems require two different DNA binding proteins
flanking a sequence of interest, each with a C-terminal
FokI nuclease module. As a result these methods have not
been widely adopted by the plant research community.
from
>Belhaj K et al., (2013).
>Plant genome editing made easy: targeted mutagenesis in model and crop
plants using the CRISPR/Cas >system.
>Plant Methods. 9: 39. [Epub ahead of print]
>http://www.ncbi.nlm.nih.gov/pubmed/24112467
or
HTTP double dot //www.plantmethods.com/content/pdf/1746-4811-9-39.pdf
more pls go to,
>Jiang W et al., (2013).
>Demonstration of CRISPR/Cas9/sgRNA-mediated targeted gene modification in
Arabidopsis, tobacco, >sorghum and rice.
>Nucleic Acids Res. 41: e188.
>Abstract
>The type II CRISPR/Cas system from Streptococcus pyogenes and its
simplified derivative, the Cas9/single >guide RNA (sgRNA) system, have
emerged as potent new tools for targeted gene knockout in bacteria, >yeast,
fruit fly, zebrafish and human cells. Here, we describe adaptations of these
systems leading to >successful expression of the Cas9/sgRNA system in two
dicot plant species
below ignored
http://www.ncbi.nlm.nih.gov/pubmed/23999092
or
HTTP double dot//nar.oxfordjournals.org/content/early/2013/08/31/nar.gkt780.
full.pdf
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