请教1 Ligation reaction question - 未名空间MITBBS历史存档

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请教1 Ligation reaction question

请教1 Ligation reaction question# Biology - 生物学

w*o2017-08-11 07:08

1 楼

We can add a 5'-PO4-DNA (Single Stranded) to the 3'-end of a ssRNA using T4
RNA ligase I, but cannot add a 5'-PO4-RNA (single Stranded) to 3'-end of
ssDNA.
Highly appreciated any suggestion on how to add a ssRNA to a ssDNA. Thanks a
lot

t*a2017-08-11 07:08

2 楼

Try this?
https://www.neb.com/protocols/2014/10/27/ligation-of-a-dna-or-rna-oligo-to-
single-stranded-rna-using-t4-rna-ligase-1

T4
a

【在 w***o 的大作中提到】

: We can add a 5'-PO4-DNA (Single Stranded) to the 3'-end of a ssRNA using T4
: RNA ligase I, but cannot add a 5'-PO4-RNA (single Stranded) to 3'-end of
: ssDNA.
: Highly appreciated any suggestion on how to add a ssRNA to a ssDNA. Thanks a
: lot

w*o2017-08-11 07:08

3 楼

We need add 5'-PO4-RNA to 3'-end of ssDNA
Not adding DNA/RNA to ssRNA

【在 t*****a 的大作中提到】

: Try this?
: https://www.neb.com/protocols/2014/10/27/ligation-of-a-dna-or-rna-oligo-to-
: single-stranded-rna-using-t4-rna-ligase-1
:
: T4
: a

w*o2017-08-11 07:08

4 楼

We tried it, didnot work

【在 t*****a 的大作中提到】

: Try this?
: https://www.neb.com/protocols/2014/10/27/ligation-of-a-dna-or-rna-oligo-to-
: single-stranded-rna-using-t4-rna-ligase-1
:
: T4
: a