r*e
2 楼
现在的老师越来越不好当了.jpg
i*h
3 楼
看了一张样片
h*9
4 楼
A question about making knock-in mice through traditional homologous
recombination---After identifying the positive ES clones by southern blot
analysis, do people usually sequence the whole new allele from 5' arm to
the end of 3' arm? Assume that the targeting vector has been fully sequenced
before electroporation.
Thanks.
recombination---After identifying the positive ES clones by southern blot
analysis, do people usually sequence the whole new allele from 5' arm to
the end of 3' arm? Assume that the targeting vector has been fully sequenced
before electroporation.
Thanks.
w*x
5 楼
here's the original version.
t*t
6 楼
链接?
j*n
7 楼
sequenced
反正我们不做 有概率也及其小 如果是点突变敲入倒是可以测序确认一下
【在 h****9 的大作中提到】
: A question about making knock-in mice through traditional homologous
: recombination---After identifying the positive ES clones by southern blot
: analysis, do people usually sequence the whole new allele from 5' arm to
: the end of 3' arm? Assume that the targeting vector has been fully sequenced
: before electroporation.
: Thanks.
L*n
10 楼
Sometimes the ends can be screwed up during recombination, so I would PCR
the arms out using external/internal primers and sequence then, just to be
safe.
the arms out using external/internal primers and sequence then, just to be
safe.
s*e
11 楼
哈哈
h*k
13 楼
at 1960, f means fantastic
i*h
14 楼
我真是觉得十年前的 4Mega Pixel Canon G2足够了
现在每张照片拍那么大文件基本也就是在计算机上看
好的低光和快速对焦实用多了
没有这些拍娃还是得上单反
现在每张照片拍那么大文件基本也就是在计算机上看
好的低光和快速对焦实用多了
没有这些拍娃还是得上单反
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