2 links of EB12 recommendation letters(ZZ) - 未名空间MITBBS历史存档

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2 links of EB12 recommendation letters(ZZ)

2 links of EB12 recommendation letters(ZZ)# Chemistry - 化学

y*n2011-12-04 08:12

1 楼

以前的信用卡是BoA的，不好用，所以申请了两个amex，一个是fidelity的，一个是
costco的，都批准了。
几个问题，
一，我同时用两个好？还是只保留一个？觉得两个没有必要，是不是fidelity的好一点
？2％的cashback
二，是不是还是要保留一个visa的信用卡？
三，申请了一个信用卡，批准了，但是不激活，取消，对信用纪录有影响么。
谢谢了。

s*a2011-12-04 08:12

2 楼

【以下文字转载自 Travel 讨论区】
发信人: synaesthesia (Pirate), 信区: Travel
标题: 版上有人去过 Lebanon 吗？
发信站: BBS 未名空间站 (Thu Nov 3 18:31:49 2011, 美东)
签证怎么申请？我有现成的 h1b 回美 visa，回美不会有什么特别的问题吧？
公司要派我过去出差，急问!

Y*H2011-12-04 08:12

3 楼

昨天在sc 1打了3盘,第三盘一直说我hack,最后输急眼直接把我drop hack了 -,-

f*c2011-12-04 08:12

4 楼

4月17日晚21：10，《奔跑吧兄弟》第二季回归。这一次的“泥潭大战”，堪称上一季舟山岛那一期的升级版。因为上一次还只是考验他们在泥潭里的行走和奔跑，这一次却是要上演极为惨烈的“贴身肉搏”。向来以霸气著称的范爷，这次的表现让所有的兄弟都对她刮目相看：真的是太拼了!

l*n2011-12-04 08:12

5 楼

http://blog.mixu.net/2011/02/01/understanding-the-node-js-event

c*y2011-12-04 08:12

6 楼

好像重复性很不好。efficiency 不同时间做差别很大。大家是怎样在这方面控制质量
的？有什么窍门分享一下好吗？多谢

s*u2011-12-04 08:12

7 楼

发信人: shmu (shmu), 信区: Postdoc
标题: 2 links of EB12 recommendation letters
发信站: BBS 未名空间站 (Sun Dec 4 07:11:55 2011, 美东)
http://www.mitbbs.com/article_t/EB23/31506251.html
http://www.mitbbs.com/article_t/Immigration/32152715.html

y*n2011-12-04 08:12

8 楼

up thx

【在 y*******n 的大作中提到】

: 以前的信用卡是BoA的，不好用，所以申请了两个amex，一个是fidelity的，一个是
: costco的，都批准了。
: 几个问题，
: 一，我同时用两个好？还是只保留一个？觉得两个没有必要，是不是fidelity的好一点
: ？2％的cashback
: 二，是不是还是要保留一个visa的信用卡？
: 三，申请了一个信用卡，批准了，但是不激活，取消，对信用纪录有影响么。
: 谢谢了。

b*k2011-12-04 08:12

9 楼

huk是谁
bso意识好得map hack一般

【在 Y*H 的大作中提到】

: 昨天在sc 1打了3盘,第三盘一直说我hack,最后输急眼直接把我drop hack了 -,-

a*o2011-12-04 08:12

10 楼

Do you mean technical replicates or biological replicates?
Technical replicates should be very similar. It is more likely that your
biological samples are not very similar. rtPCR itself should be very robust
unless you are dancing on the edge of its detection limit (>30 or >35 cycles
).

P*x2011-12-04 08:12

11 楼

都留着呗，都是好卡，又没年费
是
有

【在 y*******n 的大作中提到】

Y*H2011-12-04 08:12

12 楼

sc2的pro,sc1以前应该是B的水平.
我打得很菜...是他太弱了- -

【在 b**k 的大作中提到】

: huk是谁
: bso意识好得map hack一般

c*y2011-12-04 08:12

13 楼

robust
cycles

【在 a****o 的大作中提到】

: Do you mean technical replicates or biological replicates?
: Technical replicates should be very similar. It is more likely that your
: biological samples are not very similar. rtPCR itself should be very robust
: unless you are dancing on the edge of its detection limit (>30 or >35 cycles
: ).

y*n2011-12-04 08:12

14 楼

谢谢回答

【在 P******x 的大作中提到】

:
: 都留着呗，都是好卡，又没年费
: 是
: 有

t*t2011-12-04 08:12

15 楼

没准是马甲

c*y2011-12-04 08:12

16 楼

I mean I am trying to determine primer efficiency using the same batch of
cDNA at different days. They could be vary from 50% to 90%. Based on Qiagen
qPCR protocol, all primer efficiency should be above 83% (the corresponding
slope is less than 3.8).
Every time I did serial dilution, R^2 is pretty good; but slope number
differs markedly time by time.

robust
cycles

【在 a****o 的大作中提到】

h*02011-12-04 08:12

17 楼

cllbso

【在 Y*H 的大作中提到】

: 昨天在sc 1打了3盘,第三盘一直说我hack,最后输急眼直接把我drop hack了 -,-

n*12011-12-04 08:12

18 楼

有的时候定量确实不太稳定，我觉得大多数是加样的误差，自己多练习吧。

K*a2011-12-04 08:12

19 楼

da pp!

【在 Y*H 的大作中提到】

: 昨天在sc 1打了3盘,第三盘一直说我hack,最后输急眼直接把我drop hack了 -,-

h*n2011-12-04 08:12

20 楼

产物跑个胶看看?

S*e2011-12-04 08:12

21 楼

no way LOL

c*y2011-12-04 08:12

22 楼

产物不是问题，问题是线性回归的斜率的不确定性

【在 h********n 的大作中提到】

: 产物跑个胶看看?

Y*H2011-12-04 08:12

23 楼

liquid.huk on useast. It's an old account.
He's good just out of shape.
"
He was around B- to C+ (Edited for accuracy) on ICcup BW for a while, i know in an interview a while back he stated that he was rather good at BW and played a lot just never made a big splash in the competitive scene to get noticed by large numbers of people.
Edit: Link Huk Interview
"Zutazuta: Can you give us a brief overview of your gaming career? For example what other games have you played and how did you fair? Any other games that you enjoy outside of StarCraft 2?
HuK: Well I played a lot of games but I guess just talk about ones I took semi-seriously. Starcraft Brood War I was "semi-sponsored" and "hired" to play for certain teams/smurf. But I was never in the spotlight as some of the other Starcraft Brood War NA players, although I felt I was pretty good. I also played Company of Heroes a WWII RTS game which is really great but not very popular, not supported well, and generally dieing now. I was pretty good at this game as well but because it had such a small community there was never really any E-sports dreams for it. Other then that I guess at times I was competitive at DoTA and Hon too but never took it to the next level."
Other Interview mentions time in "Keeper" ESL interview
"Were you ever competitive in Brood War? How did that go?
Not really in the sense of most people who are known within the community. I was semi sponsored in keeper- (getting paid a salary) and I did get money to smurf for a lot of others teams like excellos, x17, etc. in clan wars. Other then that I definitely wasn't bad just never really cared about the "credit".
"

【在 t******t 的大作中提到】

: 没准是马甲

c*y2011-12-04 08:12

24 楼

产物不是问题，问题是线性回归的斜率的不确定性

【在 h********n 的大作中提到】

: 产物跑个胶看看?

s*i2011-12-04 08:12

25 楼

huk和boxer打都要靠Jessica帮忙

h*n2011-12-04 08:12

26 楼

你这个实验是要做标准曲线然后测样品绝对浓度?

c*y2011-12-04 08:12

27 楼

不是绝对浓度，仍然是相对浓度。但是需要知道primer的efficiency.
个人感觉，technical replicate也不是100%。不知有没有什么加样技巧什么的。我一
般是准备mastermix (Superarray realtime PCR mix+H2O+primer),然后在PCR plate的
well 里与DNA template混合。

【在 h********n 的大作中提到】

: 你这个实验是要做标准曲线然后测样品绝对浓度?

A*y2011-12-04 08:12

28 楼

First, when you do your titration, do you know your absolute cDNA
concentration? or every time you use different concentration? What kind of
water are you using? Are you using a filtered tip? What's your cycle like
and how's the melt?
I don't think you have to prep the PCR on a cold plate since you are doing
one step but maybe is ideal to keep it a 4 degree.

n*e2011-12-04 08:12

29 楼

我在用同一对引物的时候，各次差别不大，也就5个百分点左右。但是不同的引物效率
差别很大。最近也没集中精力分析这个问题。不过还是先看看手册找找效率低的原因。
有一点可能还是要留神，就是那个决定Ct的threshold，不过既然你的线性比较好，这
也不是主要原因。

【在 c***y 的大作中提到】

: 好像重复性很不好。efficiency 不同时间做差别很大。大家是怎样在这方面控制质量
: 的？有什么窍门分享一下好吗？多谢

c*y2011-12-04 08:12

30 楼

DNA concentration is determined by Nanodrop. And I compared the efficiency
from same batch of cDNA.
I used DNase/RNase free water order from some company (don't remember
exactly since I am not at lab), and filtered tip.
The cycle number is between 20 to 25, depends on the primer set. The melt
curves looked fine.
Why do you think 4 degree prep is ideal? Thanks

of
like

【在 A******y 的大作中提到】

: First, when you do your titration, do you know your absolute cDNA
: concentration? or every time you use different concentration? What kind of
: water are you using? Are you using a filtered tip? What's your cycle like
: and how's the melt?
: I don't think you have to prep the PCR on a cold plate since you are doing
: one step but maybe is ideal to keep it a 4 degree.