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千青为了一块骨头放弃自由
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千青为了一块骨头放弃自由# Chemistry - 化学
c*u
1
我和ld只有两张Amazon的chase的卡。2%的point,在gas,restaurant等,3%的point在
amazon。
就一张credit card好像有点太少了。所以打算每人再申请2-3张。
请问怎样组合比较好呢?
肯定要打算申请的是要买机票用的,不知道UA的chase和citi的AA,哪个bonus更大一些?
其次就是吃饭。不知道申请哪家可以在restaurant或者grocery store上拿到3%以上的
discount?
接着就是ebiz。总在网上买电子产品,所以不知道哪个卡更适合ebiz?
然后手里还有个firestone的credit card,很早之前被同学误导申请的。基本上一直不
怎么用。这个对我的credit是有影响的。所以不知道cancel掉影响大还是留着影响大?
avatar
x*c
2
http://forum.powerapple.com/modules.php?
name=forum&file=viewtopic&forum=20&folder=a&topic=18198
这个贴,和作者的blog,太强大了。
印象中这个版不需要注册才能看。不过这里给出作者的blog,所有的下载链接都是来自作者的blog,不需要上苹果网
http://www.devan-z.us/category/photography/
很多movie的教程下载,覆盖从基本曝光,flash,风景,人像,后期等各个方面。主要
集中在
Nikon和Canon两家。
经常有更新。
部分内容预览
最新日志
Topaz Adjust HDR 3.2.1 (32/64)
OnOne Software FocalPoint v1.1
All Nik Software Plug-in Windows
Kelby - Digital SLR Basics
Portrait Lighting Techniques with Bobbi Lane
热门日志
Strobist Lighting Seminar DVD
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G*G
3
i am asking some questions about the principle of pyrosequencing.
there are four types of deoxynucleotide triphosphates (dNTP).
each time one of them will be incorporated with the DNA strand.
but we don't know what type of nucleotide is in the DNA strand.
My question is the following.
1) Do we add all the four dNTPs to the reaction each time?
by 'each time' I mean each position of unknown nucleotide in the DNA strand.
2) or we add dATP, then dGTP, then dCTP, then dTTP each time?
3) do we need to clean the other three dNTPs each time when
one of the four dNTPs is incorporated with the DNA strand?
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e*s
4
这才是真正的卢梭!20年后估计混的连个众人都不如!
avatar
n*e
5
买机票,买电子产品都可以申请 AMEX SPG。这个卡可以把点分配到多个航空公司,有
灵活性。但确缺点是开户的bonus太少了点,不过你6个月可以用到$15000,有额外的
15000点,这样的话很划算。 UA chase的3万点也不错
你是学生吗?吃饭可以弄 citi forward
或者是cosco会员的话可以申请 AMEX cosco trueEarning
可以弄张 discover more或者chase freedom,会有5%的cashback rotate
最后你的firestone的卡如果不是你最早的信用卡,关掉影响不大。

些?

【在 c*******u 的大作中提到】
: 我和ld只有两张Amazon的chase的卡。2%的point,在gas,restaurant等,3%的point在
: amazon。
: 就一张credit card好像有点太少了。所以打算每人再申请2-3张。
: 请问怎样组合比较好呢?
: 肯定要打算申请的是要买机票用的,不知道UA的chase和citi的AA,哪个bonus更大一些?
: 其次就是吃饭。不知道申请哪家可以在restaurant或者grocery store上拿到3%以上的
: discount?
: 接着就是ebiz。总在网上买电子产品,所以不知道哪个卡更适合ebiz?
: 然后手里还有个firestone的credit card,很早之前被同学误导申请的。基本上一直不
: 怎么用。这个对我的credit是有影响的。所以不知道cancel掉影响大还是留着影响大?

avatar
G*G
6
My English may not be accurate. so I try it again.
I am asking about the principle of pyrosequencing.
1) when testing each base of sequence, are all the four dNTPs added
at the same time or sequently?
2) are the four dNTPs added to reaction each time of testing the base of
sequence or are they added to reaction just once during the whole sequencing
for all bases.
3) if the answer to the question 2 is 'each time of testing the base of
sequence', do we need to clean each time?

strand.

【在 G***G 的大作中提到】
: i am asking some questions about the principle of pyrosequencing.
: there are four types of deoxynucleotide triphosphates (dNTP).
: each time one of them will be incorporated with the DNA strand.
: but we don't know what type of nucleotide is in the DNA strand.
: My question is the following.
: 1) Do we add all the four dNTPs to the reaction each time?
: by 'each time' I mean each position of unknown nucleotide in the DNA strand.
: 2) or we add dATP, then dGTP, then dCTP, then dTTP each time?
: 3) do we need to clean the other three dNTPs each time when
: one of the four dNTPs is incorporated with the DNA strand?

avatar
L*r
7


【在 e***s 的大作中提到】
: 这才是真正的卢梭!20年后估计混的连个众人都不如!
avatar
i*7
8
另外这几张卡里面哪个最容易申啊...是学生

【在 n*********e 的大作中提到】
: 买机票,买电子产品都可以申请 AMEX SPG。这个卡可以把点分配到多个航空公司,有
: 灵活性。但确缺点是开户的bonus太少了点,不过你6个月可以用到$15000,有额外的
: 15000点,这样的话很划算。 UA chase的3万点也不错
: 你是学生吗?吃饭可以弄 citi forward
: 或者是cosco会员的话可以申请 AMEX cosco trueEarning
: 可以弄张 discover more或者chase freedom,会有5%的cashback rotate
: 最后你的firestone的卡如果不是你最早的信用卡,关掉影响不大。
:
: 些?

avatar
x*m
9
http://en.wikipedia.org/wiki/Pyrosequencing
"The template DNA is immobile, and solutions of A, C, G, and T nucleotides are added and removed after the reaction, sequentially. Light is produced only when the nucleotide solution complements the first unpaired base of the template. The sequence of solutions which produce chemiluminescent signals allows the determination of the sequence of the template."

strand.

【在 G***G 的大作中提到】
: i am asking some questions about the principle of pyrosequencing.
: there are four types of deoxynucleotide triphosphates (dNTP).
: each time one of them will be incorporated with the DNA strand.
: but we don't know what type of nucleotide is in the DNA strand.
: My question is the following.
: 1) Do we add all the four dNTPs to the reaction each time?
: by 'each time' I mean each position of unknown nucleotide in the DNA strand.
: 2) or we add dATP, then dGTP, then dCTP, then dTTP each time?
: 3) do we need to clean the other three dNTPs each time when
: one of the four dNTPs is incorporated with the DNA strand?

avatar
n*e
10
从discover student
BOA student
citi forward 开始

【在 i*****7 的大作中提到】
: 另外这几张卡里面哪个最容易申啊...是学生
avatar
u*a
11
dATP,dCTP,dGTP,dTTP are manually added into four different catridges. During
the reaction, 4 dNTPS are sequentially added into the reaction system. The
machine will detect which one is complement with the template, then show it
up.
avatar
i*7
12
有一张capital one的用了半年还是只能申学生卡吗?谢谢

【在 n*********e 的大作中提到】
: 从discover student
: BOA student
: citi forward 开始

avatar
b*8
13
可以考虑american expree blue or spg
avatar
n*e
14
嗯,那上面说的那些都可以试试了
但是这些都没有特别准则的,不一定条件好就申得到,条件不好就申不到
只有试了才知道
但最好半年集中试一批

【在 i*****7 的大作中提到】
: 有一张capital one的用了半年还是只能申学生卡吗?谢谢
avatar
s*g
15
都可以尝试
但有时需要一年以上信用记录
学生卡相对好申请 用起来和普通卡没啥大区别

【在 i*****7 的大作中提到】
: 有一张capital one的用了半年还是只能申学生卡吗?谢谢
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