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杂志介绍
http://www.rsc.org/publishing/journals/MB/about.asp
Impact factor: 3.859
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你的名字，你的email，你的专业特长关键词（3－5个即可），你的发表文章。
为了你省事，你可以copy paste你的resume也可以
希望不同专业都来试试，“分子生物系统”杂志算是交叉学科杂志。
谢谢！
稿件摘要：
TITLE: Noninvasive measurement of respiratory activity of mouse embryoid
bodies and its correlation with undifferentiation/differentiation markers
ABSTRACT: Mouse embryoid bodies (mEBs) were evaluated in detail on the basis
of respiratory activity and high-throughput quantitative reverse
transcription-PCR (RT-qPCR) analysis. The hanging drop culture method was
applied to prepare various sizes of mEBs ranging from 100 to 250 μm in
radius by causing the aggregation of embryonic stem cells. The respiratory
activity of individual mEBs was noninvasively measured using scanning
electrochemical microscopy in a cone-shaped microwell. For gene expression
analysis, we used 48.48 Dynamic Array chips (Fluidigm) integrating
microfluidic circuits, which allowed high-throughput qPCR analysis in
parallel. The respiratory activity of the mEBs that were cultured for 1 to 6
days could predict the gene expression of undifferentiation and
differentiation markers. However, the sizes of the mEBs could also predict
the gene expression of the undifferentiation/differentiation markers because
the radii of the mEBs increased by more than 2-fold after incubation in
hanging drop culture for 6 days. Next, mEBs with identical sample sizes were
evaluated for respiratory activity and gene expression. For mBs cultured at
1500 cells/droplet for 3 days, the respiratory activity was negatively
correlated with the expression levels of pluripotent markers such as Nanog
and Sox2. Many differentiation markers were positively correlated with the
respiratory activity, although it was difficult to predict the direction of
the differentiation based on the respiratory activity at the present stage.
Noninvasively measured respiratory activities of individual mEBs samples may
be used to select a specific cellular group with a homogeneous nature that
cannot be obtained from morphological observations.
http://www.rsc.org/publishing/journals/MB/about.asp
Impact factor: 3.859
劳驾站内信发给我：
你的名字，你的email，你的专业特长关键词（3－5个即可），你的发表文章。
为了你省事，你可以copy paste你的resume也可以
希望不同专业都来试试，“分子生物系统”杂志算是交叉学科杂志。
谢谢！
稿件摘要：
TITLE: Noninvasive measurement of respiratory activity of mouse embryoid
bodies and its correlation with undifferentiation/differentiation markers
ABSTRACT: Mouse embryoid bodies (mEBs) were evaluated in detail on the basis
of respiratory activity and high-throughput quantitative reverse
transcription-PCR (RT-qPCR) analysis. The hanging drop culture method was
applied to prepare various sizes of mEBs ranging from 100 to 250 μm in
radius by causing the aggregation of embryonic stem cells. The respiratory
activity of individual mEBs was noninvasively measured using scanning
electrochemical microscopy in a cone-shaped microwell. For gene expression
analysis, we used 48.48 Dynamic Array chips (Fluidigm) integrating
microfluidic circuits, which allowed high-throughput qPCR analysis in
parallel. The respiratory activity of the mEBs that were cultured for 1 to 6
days could predict the gene expression of undifferentiation and
differentiation markers. However, the sizes of the mEBs could also predict
the gene expression of the undifferentiation/differentiation markers because
the radii of the mEBs increased by more than 2-fold after incubation in
hanging drop culture for 6 days. Next, mEBs with identical sample sizes were
evaluated for respiratory activity and gene expression. For mBs cultured at
1500 cells/droplet for 3 days, the respiratory activity was negatively
correlated with the expression levels of pluripotent markers such as Nanog
and Sox2. Many differentiation markers were positively correlated with the
respiratory activity, although it was difficult to predict the direction of
the differentiation based on the respiratory activity at the present stage.
Noninvasively measured respiratory activities of individual mEBs samples may
be used to select a specific cellular group with a homogeneous nature that
cannot be obtained from morphological observations.
