Re: question about cloning from BAC# Biology - 生物学
l*k
1 楼
how about do southern to get physical restriction map(if you have probe for the
gene you want?)
Actually the question you asked is weird, to me :)
if you want to do funsion construct for expression, should you use cDNA??? why
you bother to go after BAC, I think they are all genomic DNA
for expression, the best system I ever used is the PET30(a,b,c) system, all my
proteins got very strong expression, HIS6 helps to purify protein using Ni+
column, thrombin can be used to remove the fursion part fr
gene you want?)
Actually the question you asked is weird, to me :)
if you want to do funsion construct for expression, should you use cDNA??? why
you bother to go after BAC, I think they are all genomic DNA
for expression, the best system I ever used is the PET30(a,b,c) system, all my
proteins got very strong expression, HIS6 helps to purify protein using Ni+
column, thrombin can be used to remove the fursion part fr