我来贴一个全一点的细胞分离方法的LIST# Biology - 生物学
r*o
1 楼
如果前面那位兄弟只是选克隆的话(比如做stable transfected cell line)，稀释细胞
培养，然后慢慢挑就是。如果要直接从一大堆细胞选出一个符合要求的细胞，方法总结如
下。
From
Culture of animal cells: a manual of basic technique (4th edition)
(1)Sedimentation velocity at 1g
* based on cell size
*Custom-made separating funnel and baffles
*Simple technique but not very high resolution or yield
(2) Isopyknic sedimentation
* based on cell density
*centrifuge
* simple and rapid
(3) MACS
* Surface antibodies
* simple magnet and flow chamber
培养，然后慢慢挑就是。如果要直接从一大堆细胞选出一个符合要求的细胞，方法总结如
下。
From
Culture of animal cells: a manual of basic technique (4th edition)
(1)Sedimentation velocity at 1g
* based on cell size
*Custom-made separating funnel and baffles
*Simple technique but not very high resolution or yield
(2) Isopyknic sedimentation
* based on cell density
*centrifuge
* simple and rapid
(3) MACS
* Surface antibodies
* simple magnet and flow chamber