Re: GST fusion protein-Thanks, buddy, but you guys misunderstood# Biology - 生物学
r*r
1 楼
1. maybe the problem is induction.
since i don't know the induction condition you use, here is my induction
condition for the protein I tried.(23 du, 2-3hrs) if still not good, try
18 du.
The proteolysis is inherent in GST fusion protein preps. You always get a 30
Kda form which is GST. The other problem about GST is that it is easy to form
dimer, especially the protein you work on is also easy to form dimer.
2. If you can not detect your protein in lysate, try to seqence your whole
vector. I kn
since i don't know the induction condition you use, here is my induction
condition for the protein I tried.(23 du, 2-3hrs) if still not good, try
18 du.
The proteolysis is inherent in GST fusion protein preps. You always get a 30
Kda form which is GST. The other problem about GST is that it is easy to form
dimer, especially the protein you work on is also easy to form dimer.
2. If you can not detect your protein in lysate, try to seqence your whole
vector. I kn