Re: 请教：GST fusion protein# Biology - 生物学
s*y
1 楼
In my own experience, GST is not a good tag because such reason:
1. the GST is on the C term, so , even the protein is not completely
expressed, the GST is still there.
2. GST has a tendency to form dimer (just like what others said)
In your case, it is very likely your protein wasn't expressed correctly.
You can try to induce it by a lower dose of IPTG. In our lab's experience,
sometime it helps to express big proteins. It is also a possibility that you
may introduce a stop codon inside your pr
1. the GST is on the C term, so , even the protein is not completely
expressed, the GST is still there.
2. GST has a tendency to form dimer (just like what others said)
In your case, it is very likely your protein wasn't expressed correctly.
You can try to induce it by a lower dose of IPTG. In our lab's experience,
sometime it helps to express big proteins. It is also a possibility that you
may introduce a stop codon inside your pr