Re: GST-Fusion 蛋白提纯求助!!!# Biology - 生物学
g*s
1 楼
if the protein is purified as GDT-fusion, you must have glutathione in the
prep, then depending on your subsequant application, you may want to dialyze
it using whatever buffer you used to purify it minus glutathione.
the storage question is trickier. if the purified protein is stabel, you can
certainly keep it in -80, but some of them are finicky. temperature shift may
cause aggregation and precipitation after freeze. my feeling is, in protein
manupilation, try to keep as little additives such
prep, then depending on your subsequant application, you may want to dialyze
it using whatever buffer you used to purify it minus glutathione.
the storage question is trickier. if the purified protein is stabel, you can
certainly keep it in -80, but some of them are finicky. temperature shift may
cause aggregation and precipitation after freeze. my feeling is, in protein
manupilation, try to keep as little additives such