platinum EPS C18 analytical column with dimensions of 250mm × 4.6mm and a particle size of 5 um, UV-Vis detector at 254 nm. EDTA is the additive of the blood collection tube and 7.2mg inside the 4ml tube. Thank you...
【在 K******S 的大作中提到】 : what kind of column? : How much EDTA?
d*e
8 楼
en... Hitachi 655A-11 pump, a SIL10ADvp auto injector, a SPD-10Avp UV-Vis detector ...
【在 b******e 的大作中提到】 : 总得有测量机器吧.
b*e
9 楼
UV不确定,我们MS的是绝对不允许edta进机器的。如果能免除还是免除吧。
detector
【在 d********e 的大作中提到】 : en... : Hitachi 655A-11 pump, a SIL10ADvp auto injector, a SPD-10Avp UV-Vis detector : ...
K*S
10 楼
I think you can try it. EDTA is there just to chelating Ca ions. I don't think it will affect the hydrophobicity of the drug.
a 4ml
【在 d********e 的大作中提到】 : platinum EPS C18 analytical column with dimensions of 250mm × 4.6mm and a : particle size of 5 um, : UV-Vis detector at 254 nm. : EDTA is the additive of the blood collection tube and 7.2mg inside the 4ml : tube. : Thank you...
K*S
11 楼
It is actually quite common to load the sample with presence of EDTA for phosphopeptide detection on MS.