打抗体用的HIS-FUSION蛋白需要透析掉尿素吗？ - 未名空间MITBBS历史存档

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打抗体用的HIS-FUSION蛋白需要透析掉尿素吗？

打抗体用的HIS-FUSION蛋白需要透析掉尿素吗？# Biology - 生物学

z*32011-04-28 07:04

1 楼

爸爸2005年来美探亲。明年3月又想让爸爸去签证。爸爸今年已经73岁了。请问这种情
况可以让中信代签吗？
非常感谢！！

h*e2011-04-28 07:04

2 楼

【以下文字转载自 sysop 讨论区】
发信人: thorton (NONE), 信区: sysop
标题: Re: 3k, 破猫说你是要流量不要脸是不是真的
发信站: BBS 未名空间站 (Sun Jan 24 07:57:57 2010, 美东)
要说汉奸，谁也比不上你这种流氓id

F*62011-04-28 07:04

3 楼

请教大家，打抗体用的HIS-FUSION蛋白由于表达在包涵体里，所以纯化时加了尿素，请
问最后纯化出来的融合蛋白里含有的8M尿素需要去掉吗？
谢谢各位的回复

C*92011-04-28 07:04

4 楼

应该是不能免面签了，第一他没打过指纹，第二距他上次来已经超过5年了，得签证失
效4年以内才免面签。
如果上次成功的话，年龄不是问题。我爸妈刚拿到了，08年来的，我爸也有70了。

n*w2011-04-28 07:04

5 楼

I think you need to do dialysis.

j*g2011-04-28 07:04

6 楼

Depending on which company you use, you might not need to do that.
I purified my proteins in denatured condition and the company it is fine for
injection. So I just sent them the protein in 8M urea.
And I got quite good antibodies.
The problem is if you are going to affinity purify your antibody.
If you are, then you will need to get protein in native condition.
In my case, I lowered the IPTG concentration and lowered the temperature for
induction and only got a tiny amount of protein.
But that was enough for me to couple it to the beads and the affinity
purification worked.
And by the way the company that I used for antibody production is Cocalico.
http://www.cocalicobiologicals.com/about.html

【在 F**********6 的大作中提到】