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求subcellular fractionation 的protocol或kit推荐
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求subcellular fractionation 的protocol或kit推荐# Biology - 生物学
o*x
1
Dear Visa Officer,
My name is Myself, the son of the applicants Father and Mother, requesting
your grant for my parents’ B2 visitor visas.
My parents and I have been planning for this 3-month family reunion for a
while. We have been trying to make it easy and efficient, and it turns out
the best way is for my parents to visit me in the United States. As working
professionals, both my wife and I are quite busy and it is very hard for us
to accommodate our schedules for an international trip. In addition, our
kids are both less than three years old, and I think a 24-hour long trip
will be too demanding for them at this point. My parents understand our
situations and they would love to come to visit us.
In addition, there is no better time than now for them to visit us. My
sister just went to college last year, and now my parents have more free
time to travel around. They care about and want to know how our lives are
like in the US and have shown a great interest in a bus tour to New York,
Washington, San Francisco, Chicago, and other world-famous metropolitan
cities. To show how grateful we are for their visiting, my wife and I are
very glad to pay for all their travel expenses. Attached is a tentative
schedule for a trip to the east coast.
My parents have no intention to immigrate into the United States. In fact,
they are typical old-fashioned Chinese. They live in the same small town in
which they were born and got a huge extended family there. My father has two
brothers and three sisters, and my mother has one brother and four sisters.
My parents are closely bonded to them and their own families. They really
enjoy this big relative network in our hometown. Although traveling in other
places from time to time, they have never thought about leaving their
relatives with whom they have been living for over a half century. Please
see attached pictures for a big happy family!
This trip is just a temporary arrangement. As I have mentioned above, they
really enjoy their current life in China. Both of them have decent pension
incomes. My mother is still employed at a supermarket because she enjoys
working. Neither of them speaks English and they will definitely come back
to their hometown after this trip.
Please kindly consider their request for visitor visas. We will deeply
appreciate your great help.
Sincerely,
Myself
Enclosures:
1. Bus tour itinerary (Spring or Summer, 2013)
2. Pictures for a Big Happy Family
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s*i
2
准备换公司了,请问需要多久告诉现在公司比较好。头头是东南亚华人,做事不
professional,怕他到时候给脸色或做一些不好的事情。逼近485 (eb1a)还在
pending
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p*c
3
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s*y
4
要检验一个transcrption factor的activation。(Cn-NFAT pathway),
NFAT被去磷酸化后,从cytosol入核。所以打算提取nuclear fraction检验NFAT 蛋白水
平。
我用了pierce 的kit, 不好用, 结果不make sense。
自己试了几个protocol, 背景太高,结果也不理想。
请教做过类似实验的xdjm 推荐个protocol,或者kit也成,能提取nuclear fraction
!多谢!
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o*x
5
只有一页,是不是有点短啊?我看前几天有个MM写了好几页,分几点论述被邀请人一定
会回国。这信到底多长合适啊,麻烦过来人指点一二吧,多谢了。有没有人把签证材料
的Index也放到这信里的?
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l*c
6
2 week is standard. I told my boss and his boss in 2 weeks.
We went out for a lunch before I left.
You don't want to burn the bridge.

【在 s****i 的大作中提到】
: 准备换公司了,请问需要多久告诉现在公司比较好。头头是东南亚华人,做事不
: professional,怕他到时候给脸色或做一些不好的事情。逼近485 (eb1a)还在
: pending

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F*y
7
home button还在啊
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h*n
8
co-ask for cytoskeleton fraction
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b*a
9
我反倒觉得太长太啰嗦了,而且重点偏了,前面两大段都在写来美国的理由,反复强调
你们忙,再一提孩子,明显就是让父母来帮忙带孩子的。对比之下,后面回国的理由就
弱了很多。另外签证就那么几分钟的事,没有哪个签证官会仔细读几页纸的信,把关键
点写清楚就够了。
一家之言,祝你好运!
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T*u
10
看你们公司的policy,先去问问hr,然后和头头商量一下交接时间。大原则不要妥协,
但是要做出合作的态度,至少要表示一下“哥们,我从你的角度考虑问题,(所以你也
应如是)”。
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w*2
11
i think the video was just from shanzhai ipad, not from real ipad.
(fake ipad components)

【在 p******c 的大作中提到】

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o*x
12
多谢建议。把孩子那块儿删掉了。
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s*i
13
谢谢大家意见。请问我还有2周多的假期,大家建议怎么休假法,是先休假再提辞职,
还是提辞职了,然后去修一周的假期
还是直接领钱。好像听说美国很多州是要求公司在员工辞职的时候给假期pay的,大家
听说过这个嘛
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p*c
14
U'll see soon this Wednesday! Wanna take a bet?

【在 w********2 的大作中提到】
: i think the video was just from shanzhai ipad, not from real ipad.
: (fake ipad components)

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d*u
15
直接问公司。

【在 s****i 的大作中提到】
: 谢谢大家意见。请问我还有2周多的假期,大家建议怎么休假法,是先休假再提辞职,
: 还是提辞职了,然后去修一周的假期
: 还是直接领钱。好像听说美国很多州是要求公司在员工辞职的时候给假期pay的,大家
: 听说过这个嘛

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w*2
16
bet on these spec?
没什么太特殊得猜想呀
唯一得悬念是是否有home button,这里得是有home button 的
我觉得这个也不重要?

【在 p******c 的大作中提到】
: U'll see soon this Wednesday! Wanna take a bet?
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c*n
17
i want home button......
the appealing rumored features to me
1. HD screen (80% usage of my ipad1 is reading papers...)
2. siri
3. faster CPU
pad to me is a bookshelf and newsreader
what's ur expectation for a pad? :)

【在 w********2 的大作中提到】
: bet on these spec?
: 没什么太特殊得猜想呀
: 唯一得悬念是是否有home button,这里得是有home button 的
: 我觉得这个也不重要?

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