借人气问如何挑单细胞？ - 未名空间MITBBS历史存档

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借人气问如何挑单细胞？

借人气问如何挑单细胞？# Biology - 生物学

v*r2011-08-14 07:08

1 楼

妈妈来美国三次了。之前都是和爸爸一起，去年爸爸过世后妈妈还来过一次。今年想再
来。但是哥哥没来过，想陪/送妈妈（妈妈身体不好）坐飞机，顺便来玩2个星期。想和
妈妈一起去领馆签 (虽然妈妈的可以中信代签)希望能增加过的机率，有人有过类似的
经验吗？
是哥哥携妈妈签还是应该妈妈携哥哥签？
background: 哥哥有家室，嫂子和侄儿去年都签过来过。

W*T2011-08-14 07:08

2 楼

如何从plate挑单细胞？thanks

j*i2011-08-14 07:08

3 楼

感觉哥哥携妈妈和妈妈携哥哥没什么差别。都是在DS160上写同行人员，不存在谁携谁
哈~

M*a2011-08-14 07:08

4 楼

A long list. To save time, try google/web of knowledge,using "single cell
analysis".

【在 W*T 的大作中提到】

: 如何从plate挑单细胞？thanks

r*e2011-08-14 07:08

5 楼

Re this

【在 j**i 的大作中提到】

: 感觉哥哥携妈妈和妈妈携哥哥没什么差别。都是在DS160上写同行人员，不存在谁携谁
: 哈~

i*o2011-08-14 07:08

6 楼

http://www.nature.com/nmeth/journal/v8/n4s/full/nmeth.1557.html
The method for the isolation of individual cells can vary. Picking single
cells manually using a mouth pipette is the most straightforward option30,
41, although this can be time-consuming and technically challenging. Laser-
assisted microdissection or fluorescence-activated cell sorting can also be
used to isolate a specific subpopulation of cells based on cell-surface
markers or fluorescent reporters42, 43, 44, 45. Cells of higher plants with
cell walls are difficult to dissociate using enzymes, but isolation of
nuclei after homogenization of tissues might be effective

v*r2011-08-14 07:08

7 楼

哥哥携妈妈，签证官叫哥哥的名字，主要是哥哥回答问题？
反过来就主要是妈妈回答？
说哥哥想陪/送妈妈（妈妈身体不好）坐飞机，顺便来玩2个星期，这个理由充分吗？很
担心，要是签证官说你陪妈妈来，又没陪她走，（妈妈要待4-5个月），就不知道怎么
回答了.

W*T2011-08-14 07:08

8 楼

thanks

be
with

【在 i**o 的大作中提到】

: http://www.nature.com/nmeth/journal/v8/n4s/full/nmeth.1557.html
: The method for the isolation of individual cells can vary. Picking single
: cells manually using a mouth pipette is the most straightforward option30,
: 41, although this can be time-consuming and technically challenging. Laser-
: assisted microdissection or fluorescence-activated cell sorting can also be
: used to isolate a specific subpopulation of cells based on cell-surface
: markers or fluorescent reporters42, 43, 44, 45. Cells of higher plants with
: cell walls are difficult to dissociate using enzymes, but isolation of
: nuclei after homogenization of tissues might be effective