Exogenous plant MIR168a specifically targets mammalian LDLRAP1: evidence of cross-kingdom regulation by microRNA Lin Zhang1,*, Dongxia Hou1,*, Xi Chen1,*, Donghai Li1,*, Lingyun Zhu1,2, Yujing Zhang1, Jing Li1, Zhen Bian1, Xiangying Liang1, Xing Cai1, Yuan Yin1, Cheng Wang1, Tianfu Zhang1, Dihan Zhu1, Dianmu Zhang1, Jie Xu1, Qun Chen1, Yi Ba3, Jing Liu1, Qiang Wang1, Jianqun Chen1, Jin Wang1, Meng Wang1, Qipeng Zhang1, Junfeng Zhang1, Ke Zen1 and Chen-Yu Zhang1 1Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 22 Hankou Road, Nanjing 210093, China 2Department of Chemistry and Biology, School of Science, National University of Defense Technology, Changsha 410073, China; 3Tianjin Medical University Cancer Institute and Hospital, Huanhuxi Road, Tiyuanbei, Tianjin 300060, China Correspondence: Chen-Yu Zhang, E-mail: c*****[email protected]; Ke Zen, E-mail: k**[email protected]; Junfeng Zhang, E-mail: j*****[email protected] *These four authors contributed equally to this work. Received 11 August 2011; Revised 23 August 2011; Accepted 26 August 2011; Published online 20 September 2011. Abstract Our previous studies have demonstrated that stable microRNAs (miRNAs) in mammalian serum and plasma are actively secreted from tissues and cells and can serve as a novel class of biomarkers for diseases, and act as signaling molecules in intercellular communication. Here, we report the surprising finding that exogenous plant miRNAs are present in the sera and tissues of various animals and that these exogenous plant miRNAs are primarily acquired orally, through food intake. MIR168a is abundant in rice and is one of the most highly enriched exogenous plant miRNAs in the sera of Chinese subjects. Functional studies in vitro and in vivo demonstrated that MIR168a could bind to the human/mouse low-density lipoprotein receptor adapter protein 1 ( LDLRAP1) mRNA, inhibit LDLRAP1 expression in liver, and consequently decrease LDL removal from mouse plasma. These findings demonstrate that exogenous plant miRNAs in food can regulate the expression of target genes in mammals.
怪不得。故事本身还是比较完整的,也有很多数据,看起来就是经过了大 杂志的蹂躏的样子然后最后落入Cell Research 的。 但是这个文章影响力肯定不会小,即使他们的生理的那一部分数据做的不是特别好 (这个可能就是他们为什么被拒),但是能够证实食物的miRNA进入人体(而不是 昆虫什么的)本身就是很重要的一个结果。后面肯定会有不少人去跟进(尤其是那些 搞营养和代谢的)。所以Cell Res 算是赚到了一个重要文章。
【在 c**A 的大作中提到】 : 两年前曾投到过science, 被拒掉了. 赫赫.
T*y
36 楼
好像植物微量元素维生素也可以通过食物摄取方式进入人体,通过调控人体内靶基因表 达方式影响生理功能
a*a
37 楼
cell res经常会收这种落榜文章 Received 11 August 2011; revised 23 August 2011; accepted 26 August 2011 看这收稿速度。。 以后生物武器有的好做了 比如说, 某不点名恐怖组织偷偷向猪中转入anti pten的microRNA,然后低价倾销。。
【在 s******y 的大作中提到】 : 怪不得。故事本身还是比较完整的,也有很多数据,看起来就是经过了大 : 杂志的蹂躏的样子然后最后落入Cell Research 的。 : 但是这个文章影响力肯定不会小,即使他们的生理的那一部分数据做的不是特别好 : (这个可能就是他们为什么被拒),但是能够证实食物的miRNA进入人体(而不是 : 昆虫什么的)本身就是很重要的一个结果。后面肯定会有不少人去跟进(尤其是那些 : 搞营养和代谢的)。所以Cell Res 算是赚到了一个重要文章。
z*k
38 楼
厉害,好的开头啊。不过这里只是做了 MIR168a inhibits LDLRAP1 expression. there is gonna be a lot of work to prove cross-kingdom regulation. P.S这个方法跟 DNA interference 有什么区别? 牛人解释下
you did not read the first post? I guess they prepared this news for a long time. 该发现的潜在意义还在于:一,该研究显著地扩展了微小核糖核酸的功能;二,该研究 提出了一个极其奇妙并且创新的理念,该理念对于人类健康和代谢产生了深远的影响; 三,该研究为我们理解跨“界”(比如动植物间)的相互作用甚至是共进化(co- evolution)提供了新的线索,也为我们思考微小核糖核酸的调控作用以及思考来源于 食物、植物以及昆虫的外源性微小核糖核酸在猎物和捕食者间的相互影响中的潜在作用 开辟了新的道路;四、该研究证明植物微小核糖核酸可能是食物中的“第七种营养成分 ”(其他六种分别是水、蛋白质、脂肪酸、碳水化合物、维他命和稀有元素);五、该 研究为代谢紊乱症的发生发展提供了一种新的分子机制;六、对于中国人来说,还为在 传统的中草药中发现一类全新的活性分子提供了依据。
I think miR168a has animal/human target, which is supprising enough, not saying it travels into animal/human cell in vivo. concentration is a manipulatoble factor. LDL
it will impact toxiology as well. If the data in that CR paper is true, it will impact all of the areas on material communication between human and other organism.
【在 j*****d 的大作中提到】 : it will impact toxiology as well. : If the data in that CR paper is true, it will impact all of the areas on : material communication between human and other organism.
Functional studies in vitro and in vivo demonstrated that MIR168a could bind to the human/mouse low-density lipoprotein receptor adapter protein 1 ( LDLRAP1) mRNA, inhibit LDLRAP1 expression in liver, and consequently decrease LDL removal from mouse plasma. 对啊,这东西会升高LDL,所以最好能从大米里面knockout MIR168a。 不过反过来说呢,没准啥东西可以升高HDL?反正miRNA吃了大概没啥大事,保健品嘛, 你懂的。。。
Exogenous plant MIR168a specifically targets mammalian LDLRAP1: evidence of cross-kingdom regulation by microRNA Lin Zhang1,*, Dongxia Hou1,*, Xi Chen1,*, Donghai Li1,*, Lingyun Zhu1,2, Yujing Zhang1, Jing Li1, Zhen Bian1, Xiangying Liang1, Xing Cai1, Yuan Yin1, Cheng Wang1, Tianfu Zhang1, Dihan Zhu1, Dianmu Zhang1, Jie Xu1, Qun Chen1, Yi Ba3, Jing Liu1, Qiang Wang1, Jianqun Chen1, Jin Wang1, Meng Wang1, Qipeng Zhang1, Junfeng Zhang1, Ke Zen1 and Chen-Yu Zhang1 1Jiangsu Engineering Research Center for microRNA Biology and Biotechnology, State Key Laboratory of Pharmaceutical Biotechnology, School of Life Sciences, Nanjing University, 22 Hankou Road, Nanjing 210093, China 2Department of Chemistry and Biology, School of Science, National University of Defense Technology, Changsha 410073, China; 3Tianjin Medical University Cancer Institute and Hospital, Huanhuxi Road, Tiyuanbei, Tianjin 300060, China Correspondence: Chen-Yu Zhang, E-mail: c*****[email protected]; Ke Zen, E-mail: k**[email protected]; Junfeng Zhang, E-mail: j*****[email protected] *These four authors contributed equally to this work. Received 11 August 2011; Revised 23 August 2011; Accepted 26 August 2011; Published online 20 September 2011. Abstract Our previous studies have demonstrated that stable microRNAs (miRNAs) in mammalian serum and plasma are actively secreted from tissues and cells and can serve as a novel class of biomarkers for diseases, and act as signaling molecules in intercellular communication. Here, we report the surprising finding that exogenous plant miRNAs are present in the sera and tissues of various animals and that these exogenous plant miRNAs are primarily acquired orally, through food intake. MIR168a is abundant in rice and is one of the most highly enriched exogenous plant miRNAs in the sera of Chinese subjects. Functional studies in vitro and in vivo demonstrated that MIR168a could bind to the human/mouse low-density lipoprotein receptor adapter protein 1 ( LDLRAP1) mRNA, inhibit LDLRAP1 expression in liver, and consequently decrease LDL removal from mouse plasma. These findings demonstrate that exogenous plant miRNAs in food can regulate the expression of target genes in mammals.
怪不得。故事本身还是比较完整的,也有很多数据,看起来就是经过了大 杂志的蹂躏的样子然后最后落入Cell Research 的。 但是这个文章影响力肯定不会小,即使他们的生理的那一部分数据做的不是特别好 (这个可能就是他们为什么被拒),但是能够证实食物的miRNA进入人体(而不是 昆虫什么的)本身就是很重要的一个结果。后面肯定会有不少人去跟进(尤其是那些 搞营养和代谢的)。所以Cell Res 算是赚到了一个重要文章。
【在 c**A 的大作中提到】 : 两年前曾投到过science, 被拒掉了. 赫赫.
T*y
124 楼
好像植物微量元素维生素也可以通过食物摄取方式进入人体,通过调控人体内靶基因表 达方式影响生理功能
a*a
125 楼
cell res经常会收这种落榜文章 Received 11 August 2011; revised 23 August 2011; accepted 26 August 2011 看这收稿速度。。 以后生物武器有的好做了 比如说, 某不点名恐怖组织偷偷向猪中转入anti pten的microRNA,然后低价倾销。。
【在 s******y 的大作中提到】 : 怪不得。故事本身还是比较完整的,也有很多数据,看起来就是经过了大 : 杂志的蹂躏的样子然后最后落入Cell Research 的。 : 但是这个文章影响力肯定不会小,即使他们的生理的那一部分数据做的不是特别好 : (这个可能就是他们为什么被拒),但是能够证实食物的miRNA进入人体(而不是 : 昆虫什么的)本身就是很重要的一个结果。后面肯定会有不少人去跟进(尤其是那些 : 搞营养和代谢的)。所以Cell Res 算是赚到了一个重要文章。
z*k
126 楼
厉害,好的开头啊。不过这里只是做了 MIR168a inhibits LDLRAP1 expression. there is gonna be a lot of work to prove cross-kingdom regulation. P.S这个方法跟 DNA interference 有什么区别? 牛人解释下
you did not read the first post? I guess they prepared this news for a long time. 该发现的潜在意义还在于:一,该研究显著地扩展了微小核糖核酸的功能;二,该研究 提出了一个极其奇妙并且创新的理念,该理念对于人类健康和代谢产生了深远的影响; 三,该研究为我们理解跨“界”(比如动植物间)的相互作用甚至是共进化(co- evolution)提供了新的线索,也为我们思考微小核糖核酸的调控作用以及思考来源于 食物、植物以及昆虫的外源性微小核糖核酸在猎物和捕食者间的相互影响中的潜在作用 开辟了新的道路;四、该研究证明植物微小核糖核酸可能是食物中的“第七种营养成分 ”(其他六种分别是水、蛋白质、脂肪酸、碳水化合物、维他命和稀有元素);五、该 研究为代谢紊乱症的发生发展提供了一种新的分子机制;六、对于中国人来说,还为在 传统的中草药中发现一类全新的活性分子提供了依据。
I think miR168a has animal/human target, which is supprising enough, not saying it travels into animal/human cell in vivo. concentration is a manipulatoble factor. LDL
it will impact toxiology as well. If the data in that CR paper is true, it will impact all of the areas on material communication between human and other organism.
【在 j*****d 的大作中提到】 : it will impact toxiology as well. : If the data in that CR paper is true, it will impact all of the areas on : material communication between human and other organism.
Functional studies in vitro and in vivo demonstrated that MIR168a could bind to the human/mouse low-density lipoprotein receptor adapter protein 1 ( LDLRAP1) mRNA, inhibit LDLRAP1 expression in liver, and consequently decrease LDL removal from mouse plasma. 对啊,这东西会升高LDL,所以最好能从大米里面knockout MIR168a。 不过反过来说呢,没准啥东西可以升高HDL?反正miRNA吃了大概没啥大事,保健品嘛, 你懂的。。。