people usually get much broad pattern using X-gal staining since it is disproportionally more sensitive than to use antibdy to stain LacZ. Think this way: if antibdy works so well, why did you even bother to make LacZ reporter in the first place?
【在 s******r 的大作中提到】 : 谢谢,这个染出来跟x-gal染的pattern应该一样吧。 : : beta-gal antibody from ABCAM works. : it is chicken hosted