amino acid substitution 是怎么个原则？# Biology - 生物学
d*g
1 楼
2/6, Animal Planet,晚上8点到11点EST，广告很多，最好用DVR录下来快进着看。
虽然挺讨厌AKC的，但还是很喜欢看这个比赛，绝大多数可爱的狗狗一辈子都接触不到
，过过眼瘾。
虽然挺讨厌AKC的，但还是很喜欢看这个比赛，绝大多数可爱的狗狗一辈子都接触不到
，过过眼瘾。
f*8
2 楼
要做mutation,譬如leucine,是 mutate to Ala?
这个mutate to what aa有什么讲究？
有啥书或文章介绍这些的？推荐一下。
谢谢。
这个mutate to what aa有什么讲究？
有啥书或文章介绍这些的？推荐一下。
谢谢。
a*t
3 楼
每年在各个州都会有地区赛吧~
你查查AKC的event time ，去现场RL更过瘾~~
我去年去了，太爽了~ 狂夸狗帅，owner就给你饼干，让你RL。。。。哈哈哈
就过还有一只小比勾，因为我给husky照相不给她照，使劲在后面推我~~
你查查AKC的event time ，去现场RL更过瘾~~
我去年去了，太爽了~ 狂夸狗帅，owner就给你饼干，让你RL。。。。哈哈哈
就过还有一只小比勾，因为我给husky照相不给她照，使劲在后面推我~~
s*y
4 楼
这个大体都是经验值。
没有什么好讲究的，自己看一看侧链的结构就知道了。
【在 f*********8 的大作中提到】
: 要做mutation,譬如leucine,是 mutate to Ala?
: 这个mutate to what aa有什么讲究？
: 有啥书或文章介绍这些的？推荐一下。
: 谢谢。
没有什么好讲究的，自己看一看侧链的结构就知道了。
【在 f*********8 的大作中提到】
: 要做mutation,譬如leucine,是 mutate to Ala?
: 这个mutate to what aa有什么讲究？
: 有啥书或文章介绍这些的？推荐一下。
: 谢谢。
T*O
5 楼
Usually you want to replace an AA because it's critical in your hypothesis.
Therefore, any perturbation would generate significant consequences. And
you want that significance to be as large as it could be theoretically
allowed. Mutating Leu to Ala is of the general practice. "Ala Scan",
remember? You could swap on Ala in replace of anything. The only exception
is Ala itself, which you should replace with Gly.
Now, it's important to notice the word "general". In many cases, simply
replacing an AA with Ala would not generate any Meaningful data. For
example, if in your hypothesis a critical AA is Asp and the enzyme lost
activity when you mutate it into Ala. How much could we learn from that?
Not that much. We don't really know all consequences for Asp/Ala being at
that particular place. In addition to the popular wish that the catalytic
site was gone, It could be the mutant didn't fold properly, or, the mutant
lost rigidity (or flexibility). Simply put it this way, you couldn't and
shouldn't rely on mutation method too much. And when you use the method,
design you experiment to cover as many facades as you could.
Therefore, any perturbation would generate significant consequences. And
you want that significance to be as large as it could be theoretically
allowed. Mutating Leu to Ala is of the general practice. "Ala Scan",
remember? You could swap on Ala in replace of anything. The only exception
is Ala itself, which you should replace with Gly.
Now, it's important to notice the word "general". In many cases, simply
replacing an AA with Ala would not generate any Meaningful data. For
example, if in your hypothesis a critical AA is Asp and the enzyme lost
activity when you mutate it into Ala. How much could we learn from that?
Not that much. We don't really know all consequences for Asp/Ala being at
that particular place. In addition to the popular wish that the catalytic
site was gone, It could be the mutant didn't fold properly, or, the mutant
lost rigidity (or flexibility). Simply put it this way, you couldn't and
shouldn't rely on mutation method too much. And when you use the method,
design you experiment to cover as many facades as you could.
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