How to test if a nuclear protein is a DNA binding protein?# Biology - 生物学F*K2012-02-20 08:021 楼Thanks for your advice
F*K2012-02-20 08:023 楼At this stage I want to know if it binds to DNA.【在 o**d 的大作中提到】: 如果你知道这个蛋白可能结合到DNA上的序列,可以做EMSA。
c*r2012-02-20 08:024 楼1) FRAP to see the protein's mobility in nucleus.2) cell fractionation to see if the protein only in DNA binding fraction
v*d2012-02-20 08:025 楼EMSA 就是用来干这个的,当然只是in vitro可以参考这个:http://tools.invitrogen.com/content/sfs/manuals/mp33075.pdfin vivo 很难证明是direct binding,要是你有目的序列,可以ChIP【在 F*K 的大作中提到】: At this stage I want to know if it binds to DNA.
l*12012-02-20 08:026 楼plus if your nuclaer protein is Proline-Rich Homeodomain protein (PRH)you can try oligomeric assembly Please go to 2010 one paper://www.ncbi.nlm.nih.gov/pubmed/20675722【在 v*********d 的大作中提到】: EMSA 就是用来干这个的,当然只是in vitro: 可以参考这个:: http://tools.invitrogen.com/content/sfs/manuals/mp33075.pdf: in vivo 很难证明是direct binding,要是你有目的序列,可以ChIP