paper help!# Biology - 生物学
s*a
1 楼
自己感觉情况比较复杂,所以到版上问问大侠们.
本人情况:硕士毕业5月份,I-20到5月30号截至.有博士的I-20,8月份生效.现在在学校做
,拿钱.
现在想申请OPT不知道可不可以,请指教.
本人情况:硕士毕业5月份,I-20到5月30号截至.有博士的I-20,8月份生效.现在在学校做
,拿钱.
现在想申请OPT不知道可不可以,请指教.
s*m
2 楼
听了现场视频之后 我知道为什么了
姚贝娜 现场和录音一样 没什么差别 都是那么完美
其他歌手的歌 现场就不行了 录音都是经过了修饰 所以也都很好
没有仔细鉴别的话 姚贝娜的录制的歌和别的歌手差别没有那么大 可是现场一对比就看
出水平了
姚贝娜 现场和录音一样 没什么差别 都是那么完美
其他歌手的歌 现场就不行了 录音都是经过了修饰 所以也都很好
没有仔细鉴别的话 姚贝娜的录制的歌和别的歌手差别没有那么大 可是现场一对比就看
出水平了
r*t
3 楼
Nature. 2012 Mar 14;483(7390):434-8. doi: 10.1038/nature10895.
Intrinsic coupling of lagging-strand synthesis to chromatin assembly.
Smith DJ, Whitehouse I.
Source
Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, 1275 York
Avenue, New York, New York 10065, USA.
Abstract
Fifty per cent of the genome is discontinuously replicated on the lagging
strand as Okazaki fragments. Eukaryotic Okazaki fragments remain poorly
characterized and, because nucleosomes are rapidly deposited on nascent DNA,
Okazaki fragment processing and nucleosome assembly potentially affect one
another. Here we show that ligation-competent Okazaki fragments in
Saccharomyces cerevisiae are sized according to the nucleosome repeat. Using
deep sequencing, we demonstrate that ligation junctions preferentially
occur near nucleosome midpoints rather than in internucleosomal linker
regions. Disrupting chromatin assembly or lagging-strand polymerase
processivity affects both the size and the distribution of Okazaki fragments
, suggesting a role for nascent chromatin, assembled immediately after the
passage of the replication fork, in the termination of Okazaki fragment
synthesis. Our studies represent the first high-resolution analysis--to our
knowledge--of eukaryotic Okazaki fragments in vivo, and reveal the
interconnection between lagging-strand synthesis and chromatin assembly.
Intrinsic coupling of lagging-strand synthesis to chromatin assembly.
Smith DJ, Whitehouse I.
Source
Molecular Biology Program, Memorial Sloan-Kettering Cancer Center, 1275 York
Avenue, New York, New York 10065, USA.
Abstract
Fifty per cent of the genome is discontinuously replicated on the lagging
strand as Okazaki fragments. Eukaryotic Okazaki fragments remain poorly
characterized and, because nucleosomes are rapidly deposited on nascent DNA,
Okazaki fragment processing and nucleosome assembly potentially affect one
another. Here we show that ligation-competent Okazaki fragments in
Saccharomyces cerevisiae are sized according to the nucleosome repeat. Using
deep sequencing, we demonstrate that ligation junctions preferentially
occur near nucleosome midpoints rather than in internucleosomal linker
regions. Disrupting chromatin assembly or lagging-strand polymerase
processivity affects both the size and the distribution of Okazaki fragments
, suggesting a role for nascent chromatin, assembled immediately after the
passage of the replication fork, in the termination of Okazaki fragment
synthesis. Our studies represent the first high-resolution analysis--to our
knowledge--of eukaryotic Okazaki fragments in vivo, and reveal the
interconnection between lagging-strand synthesis and chromatin assembly.
r*t
4 楼
and this one
Wiley Interdiscip Rev RNA. 2011 Nov 15. doi: 10.1002/wrna.113. [Epub ahead
of print]
Diversity of animal small RNA pathways and their biological utility.
Okamura K.
Source
Department of Developmental Biology, Sloan-Kettering Institute, New York, NY
, USA. o******[email protected]
Abstract
Higher eukaryotes employ extensive post-transcriptional gene regulation to
accomplish fine control of gene expression. The microRNA (miRNA) family
plays important roles in the post-transcriptional gene regulation of broad
networks of target mRNA expression. Most miRNAs are generated by a conserved
mechanism involving two RNase III enzymes Drosha and Dicer. However, work
from the past few years has uncovered diverse noncanonical miRNA pathways,
which exploit a variety of other RNA processing enzymes. In addition, the
discovery of another abundant small RNA family, endogenous short interfering
RNAs (endo-siRNAs), has also broadened the catalogs of short regulatory
RNAs. This review highlights recent studies that revealed novel small RNA
biogenesis pathways, and discusses their relevance to gene regulatory
networks. WIREs RNA 2011. doi: 10.1002/wrna.113 For further resources
related to this article, please visit the WIREs website.
Wiley Interdiscip Rev RNA. 2011 Nov 15. doi: 10.1002/wrna.113. [Epub ahead
of print]
Diversity of animal small RNA pathways and their biological utility.
Okamura K.
Source
Department of Developmental Biology, Sloan-Kettering Institute, New York, NY
, USA. o******[email protected]
Abstract
Higher eukaryotes employ extensive post-transcriptional gene regulation to
accomplish fine control of gene expression. The microRNA (miRNA) family
plays important roles in the post-transcriptional gene regulation of broad
networks of target mRNA expression. Most miRNAs are generated by a conserved
mechanism involving two RNase III enzymes Drosha and Dicer. However, work
from the past few years has uncovered diverse noncanonical miRNA pathways,
which exploit a variety of other RNA processing enzymes. In addition, the
discovery of another abundant small RNA family, endogenous short interfering
RNAs (endo-siRNAs), has also broadened the catalogs of short regulatory
RNAs. This review highlights recent studies that revealed novel small RNA
biogenesis pathways, and discusses their relevance to gene regulatory
networks. WIREs RNA 2011. doi: 10.1002/wrna.113 For further resources
related to this article, please visit the WIREs website.
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