Why choose male ES cells for making knock in/out mice?# Biology - 生物学h*92012-03-28 07:031 楼Why not female? just curious. Thank you.
w*e2012-03-28 07:034 楼Make sense to me. would like to see if others have different views.【在 D*a 的大作中提到】: 最后一步是chimera公老鼠跟很多很多母老鼠交配来得到你要的老鼠。XX的细胞在公老: 鼠里面不能得到精子吧。我是这么理解的。
g*y2012-03-28 07:035 楼应该是因为male chimera are more desirable. 如果运气不好,只有一两只老鼠是chimera,或者是ES cell contribution比较少,需要筛选很多后代才能得到germlinetransmission,公老鼠breeding就比母老鼠要有优势。用来注射ES cell 的blastocysts 的性别比是 50:50,但是如果ES cellcontribution比较多的话,就很可能把本来应该发育成母老鼠的“受体” blastocysts “反转” 成公老鼠。所以得到chimera是公老鼠的概率总是比母老鼠要大。选xy ES cell唯一的drawback大概就是如果想target x-chromosome gene,得到正确克隆的概率要小些。【在 h***9 的大作中提到】: Why not female? just curious. Thank you.