adenoviral cre or lentiviral cre for mouse primary cells?# Biology - 生物学
l*h
1 楼
http://slickdeals.net/permadeal/75312/amazon.com-alfa-wireless-
才$20，万一在用的网卡挂了的话，有个备胎还是不错的
才$20，万一在用的网卡挂了的话，有个备胎还是不错的
t*i
2 楼
哪种Cre少点细胞毒性？我主要做in vitro的细胞培养。谢谢了！
l*n
3 楼
都还是g的
n*k
4 楼
it depends on the titre, infection efficiency, promoter and ur system etc..
generally speaking ad-cre can be more effective but with higher toxicity too
...I would go with lenti-cre if it is effective enough but for some stem
cells, it might not work well...
【在 t***i 的大作中提到】
: 哪种Cre少点细胞毒性？我主要做in vitro的细胞培养。谢谢了！
generally speaking ad-cre can be more effective but with higher toxicity too
...I would go with lenti-cre if it is effective enough but for some stem
cells, it might not work well...
【在 t***i 的大作中提到】
: 哪种Cre少点细胞毒性？我主要做in vitro的细胞培养。谢谢了！
t*i
5 楼
I see, got it. I'll try lenti-cre first. Thanks a lot!
too
【在 n********k 的大作中提到】
: it depends on the titre, infection efficiency, promoter and ur system etc..
: generally speaking ad-cre can be more effective but with higher toxicity too
: ...I would go with lenti-cre if it is effective enough but for some stem
: cells, it might not work well...
too
【在 n********k 的大作中提到】
: it depends on the titre, infection efficiency, promoter and ur system etc..
: generally speaking ad-cre can be more effective but with higher toxicity too
: ...I would go with lenti-cre if it is effective enough but for some stem
: cells, it might not work well...
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