I am intending to use the LY294002 for inhibit PI3K in ES cell。 Do you guys have some idea on the treatment conditions? Thanks.
d*7
2 楼
We use 20 uM, 24 hours, in cancer cell lines, not sure about ES cells.
【在 i*********0 的大作中提到】 : I am intending to use the LY294002 for inhibit PI3K in ES cell。 : Do you guys have some idea on the treatment conditions? : Thanks.
f*e
3 楼
10-20uM in mouse ES cells
i*0
4 楼
for several hours? I am intending to incubate inhibitor for several days on ES cells.